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Table 4 Primer pairs used for PCR amplification of unique and differential regions in 18 Brucella biovars. Sequences of primer pairs, which where use to PCR amplify several differential genes across the 18 Brucella biovars along with the expected amplicon size and predicted gene function.

From: Molecular targets for rapid identification of Brucella spp

Primer pair number

Forward primer ... Reverse primer

ORF name Amplicon size, bp

Gene function

  

B. abortus

B. melitensis

B. suis

 

1

TGATAGCGCCAGACAACAAC ... TGTGCCAGCTTCGTTGTAAG

BruAb1_1825 596

BMEI0205 470

BR1846 722

Immunoglobulin-binding protein EIBE

2

AAATGTCAATCTGGGCTTCG ... TATTGAAGAACTGCGCAACG

  

BRA0378 191

Hypothetical protein

3

ATTTATGTCCGTGAACTGTCCGTC ... TTGTCCGCAAAAAGTATCAAAACG

  

BRA0369 123

Hypothetical protein

4

AACTGCTGGAGATGAATCCG ... GAATGTTTGCACGCATCAAT

  

BRA0363 149

DNA-binding protein

5

CTTTACGCCCGTGTATCGAC ... CATGGGGTCCTGTGTTGAG

 

BMEI1661 321

 

Recombinase

6

TGCAGCTCACGGATAATTTG ... ACACCTTGTCCACGCTCAC

BruAb2_0168 783

  

Outermembrane transporter

7

AGCTTCTGGAGGAGGTGGAT ... GTTCCGCCTTGTGTTTCTTC

 

BMEII0827 526

BRA0439 526

Glucose-1-phosphate cytidylyltransferase

8

TCTACACCACGCTGAAGTCG ... CCGAAAGCCGATAGAGTTTG

BruAb2_1035 393

BMEII0204 162

BRA1096 393

Transcriptional regulator, GNTR family

9

TTGTTGGAAACGGCTTTGATATC ... GAAAGTACCCACCCTCGGAAAACT

BruAb1_0266 358

BMEI1681 358

 

Hypothetical protein

10

TCATGCTGTGCCTCCAATTCC ... TTGCTGAGCAGCAGCAAGAAC

BruAb1_0248 184

BMEI1699 184

 

Hypothetical protein

Control

TCAGGCGCTTATAACCGAAG ... ATCTGCGCATAGGTCTGCTT

BruAb2_0582 261

BMEII0637 261

BRA0644 261

pcaC 4-carboxymuconolactone decarboxylase