Table 2 Summary of RT-PCR results from differentiating CDSs Brucella species, grouped by differentiating island. Transcripts detected in each differentiating sequence island of B. abortus 9-941, B. melitensis 16 M and B. suis 1330, when the cell cultures were grown at 37°C for 36 hours in trypticase soy broth (Difco).
From: Molecular targets for rapid identification of Brucella spp
| Â | B. suis | B. melitensis | B. abortus | ||||
|---|---|---|---|---|---|---|---|
Location | Predicted | Observed | No Band | Predicted | Observed | No Band | Observed |
B. suis Chr. I (S1) | 4 | 4 | - | 1 | 1 | - | - |
B. suis Chr. II (S2) | 18 | 17 | 1 | - | - | - | - |
B. melitensis Chr. I (M1) | - | - | - | 1 | 1 | - | - |
B. abortus Chr. I (A1) | - | - | - | - | - | - | 1 |
B. suis + B. melitensis Chr. I (SM1) | 1 | 1 | - | 2 | 2 | - | - |
B. suis + B. melitensis Chr. II (SM2) | 25 | 16 | 9 | 24 | 6 | 18 | - |
B. suis + B. abortus Chr. I (SA1) | 11 | 3 | 8 | - | - | - | 7 |
B. suis + B. abortus Chr. II (SA2) | 11 | 7 | 4 | - | - | - | 6 |
B. melitensis + B. abortus Chr. I (MA1) | - | - | - | 30 | 21 | 9 | 23 |