|
cepIfor
|
CAGGCGGCGATAGCTTG
| |
|
cepIrev
|
CACAGATCCGAGGACATCCA
| |
|
EXcepR3
|
CGGGATCC GAGAAAGAATGGAACTGCGC
|
Bam HI
|
|
EXcepR2
|
CGGGATCC TTGCGTCAGGGTGCTTCGATG
|
Bam HI
|
|
oligonucleotides used to clone promoters
|
position of 5' basea
|
Size (bp)
|
|
aidA
|
CAGATTCAATGTCGCG
|
3:329288
|
272
|
| |
GCACATCGGTAACGCG
|
3:329016
| |
|
scpB
|
CTGCAACGAACGACGCG
|
2:1062555
|
294
|
| |
GACGGAAGGGGAAGGGC
|
2:1062261
| |
|
cepI
|
GCCTGCAGGGCACAACGACGCCTATCATGC
|
2:2087932
|
267
|
| |
GAACGAAGGTCTGCATGGATG
|
2:2088199
| |
|
PBP
|
CGTCGGGAACGAGGCCC
|
2:2983704
|
313
|
| |
CGATGGGTTGGCGGTGGG
|
2:2983391
| |
|
phuR
|
CGTGTCGATGATCCGCG
|
2:2973940
|
404
|
| |
CACAGGTGGTCTCCC
|
2:2974344
| |
|
acyltransferase
|
CGATACACTGTGAGCCG
|
2:446287
|
336
|
| |
GTCCTTCAGCACGCCG
|
2:445951
| |
|
zmpA
|
CTCGAGGCTGGCCGGTACTG
|
3:478051
|
638
|
| |
GGATCCAGACTGAAGGCGGACG
|
3:478689
| |
|
MST005
|
GCACGCCCGCGTCAGGCG
|
1:366108
|
325
|
| |
CGCAAGCCACCACTACCCC
| | |
|
MST011
|
CCTTGCTGAGATTGCCGGC
|
1:779005
|
321
|
| |
GACAGCGCGTTCACGGGCG
| | |
|
MST028
|
CGTGTCGTTGCGGCGCGC
|
1:1484174
|
451
|
| |
GTCTGGCTGTACGCACGCC
| | |
|
MST052
|
CCGTCATTTGTCGTCGGGC
|
1:3009328
|
341
|
| |
CCAGTCCATCGTGGCCGC
| | |
|
MST059
|
CGCCTTCGGCAGCCCCG
|
1:3488873
|
315
|
| |
GCTGGTCGAGCAGCAGCGG
| | |
|
MST068
|
CGTCGAGCGTCAGCTTGCGC
|
2:11203
|
325
|
| |
GGTCGAGCGTCCCGCGC
| | |
|
MST072
|
GCATCCAGCAGGCGCGC
|
2:84846
|
398
|
| |
CCGACGGGACCGCAGCCC
| | |
|
MST112
|
GCAGGTCGCCATGCCGGG
|
2:2156170
|
441
|
| |
ACCACGCGTACGCGGGC
| | |
|
Mutagenic Oligonucleotides
|
|
CepBx103
|
GCGTCTTTACGCGTCGAC CCTGTAAGAGTTACC
|
Sal I
|
|
CepBx104
|
GTCTTTACGCCGTCATATG GTAAGAGTTACCAG
|
Nde I
|
|
CepBx105
|
CGCCGTCACCCCTGCAG AGTTACCAGTTACAGG
|
PstI
|
|
CepBx106
|
GCCGTCACCCTGCTGCAG TTACCAGTTACAGG
|
Pst I
|
|
CepBx107F
|
ACGCCGTCACCCTGTAACTAGT ACCAGTTACAGGCTCCTC
|
Spe I
|
|
CepBx107R
|
GAGGAGCCTGTAACTGGTACTAGT TACAGGGTGACGGCGT
|
Spe I
|
|
CepBx108F
|
CCGTCACCCTGTAAGAGTCTAGA GTTACAGGCTCCTCGTGC
|
Xba I
|
|
CepBx108R
|
GCACGAGGAGCCTGTAACTCTAGA CTCTTACAGGGTGACGG
|
Xba I
|
|
CepBx109F
|
CACCCTGTAAGAGTTACCGTCGAC AGGCTCCTCGTGCCGCGC
|
Sal I
|
|
CepBx109R
|
GCGCGGCACGAGGAGCCTGTCGAC GGTAACTCTTACAGGGTG
|
Sal I
|
|
CepBx110F
|
CCTGTAAGAG TTACCAGTTAAGATCT CCTC GTGCCGCGCG CTG
|
Bgl II
|
|
CepBx110R
|
CAGCGCGCGG CACGAGGAGATCT TAACTGG TAACTCTTAC AGG
|
Bgl II
|
|
CepBx111F
|
AGAGTTACCAGTTACAGGGATATC GTGCCGCGCGCTGTAATG
|
Eco RV
|
|
CepBx111R
|
CATTACAGCGCGCGGCACGATATC CCTGTAACTGGTAACTCT
|
Eco RV
|
|
CepBx112F
|
GTTACCAGTTACAGGCTCCGTACG CCGCGCGCTGTAATGCAC
|
Bsi WI
|
|
CepBx112R
|
GTGCATTACAGCGCGCGGCGTACG GAGCCTGTAACTGGTAAC
|
Bsi WI
|
|
CepBx113F
|
CCAGTTACAGGCTCCTCGTCGAC CGCGCTGTAATGCACGC
|
Sal I
|
|
CepBx113R
|
GCGTGCATTACAGCGCGGTCGAC GAGGAGCCTGTAACTGG
|
Sal I
|
