Table 5 Oligonucelotide primers
Primer | Sequence | Â | Restriction Site or size of product (bp) |
|---|---|---|---|
PCR Oligonucleotides | |||
cepIfor | CAGGCGGCGATAGCTTG | Â | |
cepIrev | CACAGATCCGAGGACATCCA | Â | |
EXcepR3 | CGGGATCC GAGAAAGAATGGAACTGCGC | Bam HI | |
EXcepR2 | CGGGATCC TTGCGTCAGGGTGCTTCGATG | Bam HI | |
oligonucleotides used to clone promoters | position of 5' basea | Size (bp) | |
aidA | CAGATTCAATGTCGCG | 3:329288 | 272 |
| Â | GCACATCGGTAACGCG | 3:329016 | Â |
scpB | CTGCAACGAACGACGCG | 2:1062555 | 294 |
| Â | GACGGAAGGGGAAGGGC | 2:1062261 | Â |
cepI | GCCTGCAGGGCACAACGACGCCTATCATGC | 2:2087932 | 267 |
| Â | GAACGAAGGTCTGCATGGATG | 2:2088199 | Â |
PBP | CGTCGGGAACGAGGCCC | 2:2983704 | 313 |
| Â | CGATGGGTTGGCGGTGGG | 2:2983391 | Â |
phuR | CGTGTCGATGATCCGCG | 2:2973940 | 404 |
| Â | CACAGGTGGTCTCCC | 2:2974344 | Â |
acyltransferase | CGATACACTGTGAGCCG | 2:446287 | 336 |
| Â | GTCCTTCAGCACGCCG | 2:445951 | Â |
zmpA | CTCGAGGCTGGCCGGTACTG | 3:478051 | 638 |
| Â | GGATCCAGACTGAAGGCGGACG | 3:478689 | Â |
MST005 | GCACGCCCGCGTCAGGCG | 1:366108 | 325 |
| Â | CGCAAGCCACCACTACCCC | Â | Â |
MST011 | CCTTGCTGAGATTGCCGGC | 1:779005 | 321 |
| Â | GACAGCGCGTTCACGGGCG | Â | Â |
MST028 | CGTGTCGTTGCGGCGCGC | 1:1484174 | 451 |
| Â | GTCTGGCTGTACGCACGCC | Â | Â |
MST052 | CCGTCATTTGTCGTCGGGC | 1:3009328 | 341 |
| Â | CCAGTCCATCGTGGCCGC | Â | Â |
MST059 | CGCCTTCGGCAGCCCCG | 1:3488873 | 315 |
| Â | GCTGGTCGAGCAGCAGCGG | Â | Â |
MST068 | CGTCGAGCGTCAGCTTGCGC | 2:11203 | 325 |
| Â | GGTCGAGCGTCCCGCGC | Â | Â |
MST072 | GCATCCAGCAGGCGCGC | 2:84846 | 398 |
| Â | CCGACGGGACCGCAGCCC | Â | Â |
MST112 | GCAGGTCGCCATGCCGGG | 2:2156170 | 441 |
| Â | ACCACGCGTACGCGGGC | Â | Â |
Mutagenic Oligonucleotides | |||
CepBx103 | GCGTCTTTACGCGTCGAC CCTGTAAGAGTTACC | Sal I | |
CepBx104 | GTCTTTACGCCGTCATATG GTAAGAGTTACCAG | Nde I | |
CepBx105 | CGCCGTCACCCCTGCAG AGTTACCAGTTACAGG | PstI | |
CepBx106 | GCCGTCACCCTGCTGCAG TTACCAGTTACAGG | Pst I | |
CepBx107F | ACGCCGTCACCCTGTAACTAGT ACCAGTTACAGGCTCCTC | Spe I | |
CepBx107R | GAGGAGCCTGTAACTGGTACTAGT TACAGGGTGACGGCGT | Spe I | |
CepBx108F | CCGTCACCCTGTAAGAGTCTAGA GTTACAGGCTCCTCGTGC | Xba I | |
CepBx108R | GCACGAGGAGCCTGTAACTCTAGA CTCTTACAGGGTGACGG | Xba I | |
CepBx109F | CACCCTGTAAGAGTTACCGTCGAC AGGCTCCTCGTGCCGCGC | Sal I | |
CepBx109R | GCGCGGCACGAGGAGCCTGTCGAC GGTAACTCTTACAGGGTG | Sal I | |
CepBx110F | CCTGTAAGAG TTACCAGTTAAGATCT CCTC GTGCCGCGCG CTG | Bgl II | |
CepBx110R | CAGCGCGCGG CACGAGGAGATCT TAACTGG TAACTCTTAC AGG | Bgl II | |
CepBx111F | AGAGTTACCAGTTACAGGGATATC GTGCCGCGCGCTGTAATG | Eco RV | |
CepBx111R | CATTACAGCGCGCGGCACGATATC CCTGTAACTGGTAACTCT | Eco RV | |
CepBx112F | GTTACCAGTTACAGGCTCCGTACG CCGCGCGCTGTAATGCAC | Bsi WI | |
CepBx112R | GTGCATTACAGCGCGCGGCGTACG GAGCCTGTAACTGGTAAC | Bsi WI | |
CepBx113F | CCAGTTACAGGCTCCTCGTCGAC CGCGCTGTAATGCACGC | Sal I | |
CepBx113R | GCGTGCATTACAGCGCGGTCGAC GAGGAGCCTGTAACTGG | Sal I | |