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Table 5 Oligonucelotide primers
| Primer | Sequence | Restriction Site or size of product (bp) | |
|---|---|---|---|
| PCR Oligonucleotides | |||
| cepIfor | CAGGCGGCGATAGCTTG | ||
| cepIrev | CACAGATCCGAGGACATCCA | ||
| EXcepR3 | CGGGATCC GAGAAAGAATGGAACTGCGC | Bam HI | |
| EXcepR2 | CGGGATCC TTGCGTCAGGGTGCTTCGATG | Bam HI | |
| oligonucleotides used to clone promoters | position of 5' basea | Size (bp) | |
| aidA | CAGATTCAATGTCGCG | 3:329288 | 272 |
| GCACATCGGTAACGCG | 3:329016 | ||
| scpB | CTGCAACGAACGACGCG | 2:1062555 | 294 |
| GACGGAAGGGGAAGGGC | 2:1062261 | ||
| cepI | GCCTGCAGGGCACAACGACGCCTATCATGC | 2:2087932 | 267 |
| GAACGAAGGTCTGCATGGATG | 2:2088199 | ||
| PBP | CGTCGGGAACGAGGCCC | 2:2983704 | 313 |
| CGATGGGTTGGCGGTGGG | 2:2983391 | ||
| phuR | CGTGTCGATGATCCGCG | 2:2973940 | 404 |
| CACAGGTGGTCTCCC | 2:2974344 | ||
| acyltransferase | CGATACACTGTGAGCCG | 2:446287 | 336 |
| GTCCTTCAGCACGCCG | 2:445951 | ||
| zmpA | CTCGAGGCTGGCCGGTACTG | 3:478051 | 638 |
| GGATCCAGACTGAAGGCGGACG | 3:478689 | ||
| MST005 | GCACGCCCGCGTCAGGCG | 1:366108 | 325 |
| CGCAAGCCACCACTACCCC | |||
| MST011 | CCTTGCTGAGATTGCCGGC | 1:779005 | 321 |
| GACAGCGCGTTCACGGGCG | |||
| MST028 | CGTGTCGTTGCGGCGCGC | 1:1484174 | 451 |
| GTCTGGCTGTACGCACGCC | |||
| MST052 | CCGTCATTTGTCGTCGGGC | 1:3009328 | 341 |
| CCAGTCCATCGTGGCCGC | |||
| MST059 | CGCCTTCGGCAGCCCCG | 1:3488873 | 315 |
| GCTGGTCGAGCAGCAGCGG | |||
| MST068 | CGTCGAGCGTCAGCTTGCGC | 2:11203 | 325 |
| GGTCGAGCGTCCCGCGC | |||
| MST072 | GCATCCAGCAGGCGCGC | 2:84846 | 398 |
| CCGACGGGACCGCAGCCC | |||
| MST112 | GCAGGTCGCCATGCCGGG | 2:2156170 | 441 |
| ACCACGCGTACGCGGGC | |||
| Mutagenic Oligonucleotides | |||
| CepBx103 | GCGTCTTTACGCGTCGAC CCTGTAAGAGTTACC | Sal I | |
| CepBx104 | GTCTTTACGCCGTCATATG GTAAGAGTTACCAG | Nde I | |
| CepBx105 | CGCCGTCACCCCTGCAG AGTTACCAGTTACAGG | PstI | |
| CepBx106 | GCCGTCACCCTGCTGCAG TTACCAGTTACAGG | Pst I | |
| CepBx107F | ACGCCGTCACCCTGTAACTAGT ACCAGTTACAGGCTCCTC | Spe I | |
| CepBx107R | GAGGAGCCTGTAACTGGTACTAGT TACAGGGTGACGGCGT | Spe I | |
| CepBx108F | CCGTCACCCTGTAAGAGTCTAGA GTTACAGGCTCCTCGTGC | Xba I | |
| CepBx108R | GCACGAGGAGCCTGTAACTCTAGA CTCTTACAGGGTGACGG | Xba I | |
| CepBx109F | CACCCTGTAAGAGTTACCGTCGAC AGGCTCCTCGTGCCGCGC | Sal I | |
| CepBx109R | GCGCGGCACGAGGAGCCTGTCGAC GGTAACTCTTACAGGGTG | Sal I | |
| CepBx110F | CCTGTAAGAG TTACCAGTTAAGATCT CCTC GTGCCGCGCG CTG | Bgl II | |
| CepBx110R | CAGCGCGCGG CACGAGGAGATCT TAACTGG TAACTCTTAC AGG | Bgl II | |
| CepBx111F | AGAGTTACCAGTTACAGGGATATC GTGCCGCGCGCTGTAATG | Eco RV | |
| CepBx111R | CATTACAGCGCGCGGCACGATATC CCTGTAACTGGTAACTCT | Eco RV | |
| CepBx112F | GTTACCAGTTACAGGCTCCGTACG CCGCGCGCTGTAATGCAC | Bsi WI | |
| CepBx112R | GTGCATTACAGCGCGCGGCGTACG GAGCCTGTAACTGGTAAC | Bsi WI | |
| CepBx113F | CCAGTTACAGGCTCCTCGTCGAC CGCGCTGTAATGCACGC | Sal I | |
| CepBx113R | GCGTGCATTACAGCGCGGTCGAC GAGGAGCCTGTAACTGG | Sal I | |
