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Figure 5 | BMC Microbiology

Figure 5

From: Effect of iron on the expression of sirR and sitABC in biofilm-associated Staphylococcus epidermidis

Figure 5

RNA dot blot. RNA samples were taken from bacteria pre-incubated overnight in fRPMI and incubated together with catheter fragments in fRPMI-Fe25. In the first row, each blot contains 1 μg of total RNA, in the second row each blot contains a 1/10 dilution of 1 μg of total RNA. The probe used for the hybridization is the sitC-specific RNA probe. Lane 1: RNA from planktonic bacteria after overnight incubation in fRPMI; Lane 2: RNA from sessile bacteria grown in fRPMI-Fe25; t = 2 hrs; Lane 3: RNA from planktonic bacteria grown in fRPMI-Fe25; t = 2 hrs; Lane 4: RNA from sessile bacteria grown in fRPMI-Fe25; t = 6 hrs; Lane 5: RNA from planktonic bacteria grown in fRPMI-Fe25; t = 6 hrs;

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