Skip to main content

Table 1 Himar 1 gene interruptions that attenuate survival of B. melitensis 16 M in J774.A1 macrophages assorted according to COG‡

From: Mariner mutagenesis of Brucella melitensis reveals genes with previously uncharacterized roles in virulence and survival

Functional Class

B. melitensis (BME) locus*

Genes Identified (#)

Fraction Identified (%)

Novel Genes (#)

Target Specificity†

Intracellular trafficking, secretion

II0025, II0026, II0027, II0028, II0029, II0029/30 II0030, II0032, II0033, II0034, II0035

11

12%

3

12.8

Nucleotide transport and metabolism

I0233, I0295, I0296, I1123, I1124, I1127, I1204, I1240, I1488, I1519

10

11%

5

3.5

Signal transduction

I0066, I1296, I1327, I1807, II0011

5

5%

3

2.4

Transcription

I0304/5, I0371, I0508, I0513, I0513/4, I0731/2, I0808, I1178, I1297/8, I1364, I1647/8, II1116, II1116/7

13

14%

11

2.1

Cell wall/membrane/envelope biogenesis

I0498/9, I0997, I1302, I1326, I1393, I1413, I1414, I1415, I1416, I1426, I1427, I1886, II0380, II0472, II0899

15

16%

7

1.9

Energy production and conversion

I0972, I1749, II0428, II0429, II0759, II0760, II0761, II0762

8

8%

2

1.2

Amino acid transport and metabolism

I0025, II0039, II0040, II0285,

4

4%

1

1.0

Replication, recombination and repair

I0040, I0334, I1307, I2023, II0260

5

5%

4

0.5

Translation, ribosome structure and biogenesis

I0983, I1057, I1775/6

3

3%

3

0.4

Function unknown

I0186, I0193, I0490, I0540, I0603, I0732, II0045/6

7

7%

7

0.4

Posttranslational modification, protein turnover, chaperones

I0816, I1804,II0932

3

3%

2

0.4

Defense mechanisms

I0926

1

1%

1

0.3

General function prediction

I1143, I1282, I1487, I1499, I1867, I1894

5

5%

5

0.2

Carbohydrate transport and metabolism

I2031, II1095

2

2%

1

0.2

Lipid transport and metabolism

I1553

1

1%

 

0.1

Coenzyme transport and metabolism

I0657

1

1%

1

0.1

  1. ‡ Genes assigned to COGs that are not listed were not identified.
  2. * Numbers in bold reflect novel identification (n = 57). Those that are not bold have been identified previously using Tn5 by us and others [46]
  3. / Indicates intergenic insertion between two genes.
  4. † ts or target specificity represents the ratio of insertions per COG divided by the fraction of the genome represented by that COG.