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Table 1 Himar 1 gene interruptions that attenuate survival of B. melitensis 16 M in J774.A1 macrophages assorted according to COG‡

From: Mariner mutagenesis of Brucella melitensis reveals genes with previously uncharacterized roles in virulence and survival

Functional Class B. melitensis (BME) locus* Genes Identified (#) Fraction Identified (%) Novel Genes (#) Target Specificity†
Intracellular trafficking, secretion II0025, II0026, II0027, II0028, II0029, II0029/30 II0030, II0032, II0033, II0034, II0035 11 12% 3 12.8
Nucleotide transport and metabolism I0233, I0295, I0296, I1123, I1124, I1127, I1204, I1240, I1488, I1519 10 11% 5 3.5
Signal transduction I0066, I1296, I1327, I1807, II0011 5 5% 3 2.4
Transcription I0304/5, I0371, I0508, I0513, I0513/4, I0731/2, I0808, I1178, I1297/8, I1364, I1647/8, II1116, II1116/7 13 14% 11 2.1
Cell wall/membrane/envelope biogenesis I0498/9, I0997, I1302, I1326, I1393, I1413, I1414, I1415, I1416, I1426, I1427, I1886, II0380, II0472, II0899 15 16% 7 1.9
Energy production and conversion I0972, I1749, II0428, II0429, II0759, II0760, II0761, II0762 8 8% 2 1.2
Amino acid transport and metabolism I0025, II0039, II0040, II0285, 4 4% 1 1.0
Replication, recombination and repair I0040, I0334, I1307, I2023, II0260 5 5% 4 0.5
Translation, ribosome structure and biogenesis I0983, I1057, I1775/6 3 3% 3 0.4
Function unknown I0186, I0193, I0490, I0540, I0603, I0732, II0045/6 7 7% 7 0.4
Posttranslational modification, protein turnover, chaperones I0816, I1804,II0932 3 3% 2 0.4
Defense mechanisms I0926 1 1% 1 0.3
General function prediction I1143, I1282, I1487, I1499, I1867, I1894 5 5% 5 0.2
Carbohydrate transport and metabolism I2031, II1095 2 2% 1 0.2
Lipid transport and metabolism I1553 1 1%   0.1
Coenzyme transport and metabolism I0657 1 1% 1 0.1
  1. ‡ Genes assigned to COGs that are not listed were not identified.
  2. * Numbers in bold reflect novel identification (n = 57). Those that are not bold have been identified previously using Tn5 by us and others [46]
  3. / Indicates intergenic insertion between two genes.
  4. † ts or target specificity represents the ratio of insertions per COG divided by the fraction of the genome represented by that COG.
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