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Table 5 Comparison between ISO-IDF and real-time PCR methods on collected naturally contaminated samples

From: A robotic DNA purification protocol and real-time PCR for the detection of Enterobacter sakazakii in powdered infant formulae

   real-time PCR (C T values)    real-time PCR (C T values)
Sample number ISO 1 2 Sample number ISO 1 2
1 - - - 22 + 19.83 21.84
2 - - - 23 + 24.32 25.83
3 - - - 24 - - -
4 - - - 25 + 20.27 20.38
5 + 21 20.94 26 - - -
6 + 24.41 22.42 27 + 20.93 20.35
7 + 20.82 19.63 28 - - -
8 - - - 29 - - -
9 - - - 30 - - -
10 + 21.6 21.39 31 + 22.00 19.76
11 + 19.7 19.69 32 + 24.67 23.80
12 - - - 33 + 19.73 20.93
13 + 18.65 19.65 34 - - -
14 + 26.07 27.57 35 + 24.41 24.84
15 - - - 36 + 19.03 20.46
16 - - - 37 + 24.30 24.34
17 - - - 38 - - -
18 + 19.75 21.50 39 + 23.63 22.94
19 + 22.58 23.67 40 - - -
20 + 21.58 21.89 41 + 19.87 20.14
21 - 36.95 36.35     
  1. +: detection of E. sakazakii; -: no detection