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Table 5 Comparison between ISO-IDF and real-time PCR methods on collected naturally contaminated samples

From: A robotic DNA purification protocol and real-time PCR for the detection of Enterobacter sakazakii in powdered infant formulae

  

real-time PCR (C T values)

  

real-time PCR (C T values)

Sample number

ISO

1

2

Sample number

ISO

1

2

1

-

-

-

22

+

19.83

21.84

2

-

-

-

23

+

24.32

25.83

3

-

-

-

24

-

-

-

4

-

-

-

25

+

20.27

20.38

5

+

21

20.94

26

-

-

-

6

+

24.41

22.42

27

+

20.93

20.35

7

+

20.82

19.63

28

-

-

-

8

-

-

-

29

-

-

-

9

-

-

-

30

-

-

-

10

+

21.6

21.39

31

+

22.00

19.76

11

+

19.7

19.69

32

+

24.67

23.80

12

-

-

-

33

+

19.73

20.93

13

+

18.65

19.65

34

-

-

-

14

+

26.07

27.57

35

+

24.41

24.84

15

-

-

-

36

+

19.03

20.46

16

-

-

-

37

+

24.30

24.34

17

-

-

-

38

-

-

-

18

+

19.75

21.50

39

+

23.63

22.94

19

+

22.58

23.67

40

-

-

-

20

+

21.58

21.89

41

+

19.87

20.14

21

-

36.95

36.35

    
  1. +: detection of E. sakazakii; -: no detection