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Table 2 Sensitivity of real-time PCR for detection of a serial 10-fold diluted E. sakazakii DNA in presence of IAC

From: A robotic DNA purification protocol and real-time PCR for the detection of Enterobacter sakazakii in powdered infant formulae

 

Strain 29544

Strain ATCC 51329

Nb. of genome copies/PCR

C T value (target)

C T value (IAC)

C T value (target)

C T value (IAC)

106

19.60 ± 0.15

-

23.79 ± 0.23

-

105

23.75 ± 0.16

-

28.24 ± 0.50

-

104

27.99 ± 0.98

-

32.11 ± 0.49

-

103

32.86 ± 0.03

> 38

37.04 ± 0.71

39.44 ± 1.20

102

36.27 ± 0.68

40.85 ± 1.11

40.56 ± 0.86

41.10 ± 0.99

50

37.67 ± 0.21

40.53 ± 0.85

41.49 ± 0.75

40.32 ± 0.22

25

38.42 ± 0.46

40.24 ± 1.53

42.09 ± 0.97

41.20 ± 0.62

10

38.99 ± 1.82

37.14 ± 1.66

43.73 ± 1.41

41.29 ± 1.84

5

40.79 ± 1.41

38.77 ± 2.95

> 43.7

40.22 ± 1.77

1

> 44

40.46 ± 0.87

> 45

39.86 ± 0.27

  1. C T values are means ± standard deviation obtained for three replicates