Skip to main content

Advertisement

Table 2 Sensitivity of real-time PCR for detection of a serial 10-fold diluted E. sakazakii DNA in presence of IAC

From: A robotic DNA purification protocol and real-time PCR for the detection of Enterobacter sakazakii in powdered infant formulae

  Strain 29544 Strain ATCC 51329
Nb. of genome copies/PCR C T value (target) C T value (IAC) C T value (target) C T value (IAC)
106 19.60 ± 0.15 - 23.79 ± 0.23 -
105 23.75 ± 0.16 - 28.24 ± 0.50 -
104 27.99 ± 0.98 - 32.11 ± 0.49 -
103 32.86 ± 0.03 > 38 37.04 ± 0.71 39.44 ± 1.20
102 36.27 ± 0.68 40.85 ± 1.11 40.56 ± 0.86 41.10 ± 0.99
50 37.67 ± 0.21 40.53 ± 0.85 41.49 ± 0.75 40.32 ± 0.22
25 38.42 ± 0.46 40.24 ± 1.53 42.09 ± 0.97 41.20 ± 0.62
10 38.99 ± 1.82 37.14 ± 1.66 43.73 ± 1.41 41.29 ± 1.84
5 40.79 ± 1.41 38.77 ± 2.95 > 43.7 40.22 ± 1.77
1 > 44 40.46 ± 0.87 > 45 39.86 ± 0.27
  1. C T values are means ± standard deviation obtained for three replicates