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Table 1 Bacterial panel used to evaluate the specificity and sensitivity of the real-time PCR assay

From: A robotic DNA purification protocol and real-time PCR for the detection of Enterobacter sakazakii in powdered infant formulae

Species/Serotypes nb Strains (source) Species/Serotypes nb Strains (source)
Enterobacter sakazakii 35 ATCC 29544 (CIP) Salmonella Hadar 2 (AFSSA)
   CIP 104951 (CIP) Salmonella Typhimurium 2 (AFSSA, BgVV)
   CIP 104952 (CIP) Salmonella Typhi 1 (AFSSA)
   ES 1, ES 2, ES 5, Salmonella Kottbuss 1 (AFSSA)
   ES 9, ES 10, ES 16, Salmonella Virchow 1 (AFSSA)
   ES 17, ES 18, ES 19, Salmonella Derby 1 (AFSSA)
   ES 20, ES 23, ES 28, Salmonella Enteritidis 2 (AFSSA, BgVV)
   ES 29, ES 30, ES 32, Salmonella Bredeney 1 (BgVV)
   ES 34, ES 37, ES 40, Salmonella Agona 1 (AFSSA)
   ES 41, ES 44, ES 52, Salmonella Indiana 1 (AFSSA)
   ES 53 (IFF) Salmonella Saintpaul 1 (AFSSA)
   ES8110043 (BM) Salmonella Montevideo 1 (AFSSA)
   ES8311015 (BM) Salmonella Anatum 1 (AFSSA)
   ES8704047 (BM) Salmonella Dublin 1 (BgVV)
   ATCC 51329 (BM) Salmonella (II) 1 (BgVV)
   ES8405628 (BM) Salmonella Senftenberg 1 (AFSSA)
   ES810726 (BM) Salmonella (IIIa) 1 (AFSSA)
   HMPL118 (AFSSA) Salmonella (IIIb) 1 (BgVV)
   HMPL194 (AFSSA) Salmonella Arizonae 1 (AFSSA)
   HMPL1937 (AFSSA) Serratia fonticola 1 CIP 78.64 (CIP)
   HMPL1740 (AFSSA) Serratia liquefaciens 4 (CTSCCV, AFSSA)
Enterobacter gergoviae 1 ATCC 33028 (CIP) Serratia marcescens 3 (AFSSA)
Enterobacter hormaechei 1 ATCC 49162 (CIP) Raoultella planticola 1 CIP 81.36 (CIP)
Enterobacter kobei 1 ATCC BAA-260 (CIP) Yersinia enterocolitica 2 (AFSSA)
Enterobacter pyrinus 1 ATCC 49851 (CIP) Aeromonas putrefaciens 1 (AFSSA)
Enterobacter sp. 1 CIP 53.120 (CIP) Aeromonas hydrophila 1 (AFSSA)
Enterobacter amnigenus 4 (CTSCCV, AFSSA) Acinetobacter sp. 1 (AFSSA)
Enterobacter cloacae 18 (AFSSA) Acinetobacter baumanii 1 CIP 70.34 (CIP)
Enterobacter aerogenes 5 (AFSSA) Acinetobacter johnsonii 1 (AFSSA)
Enterobacter agglomerans 4 (AFSSA) Acinetobacter lwoffii 1 CIP 104272 (CIP)
Enterobacter intermedius 3 (AFSSA) Pseudomonas fluorescens 2 (AFSSA)
Citrobacter freundii 8 (AFSSA) Pseudomonas aeruginosa 2 (AFSSA)
Citrobacter koseri 6 i.e. ATCC 27028 (CIP, AFSSA) Stenotrophomonas maltophilia 1 (AFSSA)
Citrobacter sp. 1 (AFSSA) Campylobacter jejuni 2 CCUG 11284
Citrobacter farmeri 1 (AFSSA) Alcaligenes tolerans 1 CIP55.94 (CIP)
Escherichia coli (i.e. O86; O127:H6; O157:H7; O55; O145; O113:H2; O103) 12 i.e. NCTC12900 (AFSSA) Alcaligenes faecalis 1 CIP62.32 (CIP)
    Listeria monocytogenes 5 i.e. clip 74902 (CIP, AFSSA)
Escherichia vulneris 1 (AFSSA) Listeria innocua 1 (AFSSA)
Escherichia hermanii 1 (AFSSA) Listeria weshimeri 1 ATCC 35897
Escherichia fergusonnii 1 (AFSSA) Listeria seeligeri 1 Clip 12513 (CIP)
Buttiauxella agrestis 1 (AFSSA) Listeria ivanovii 1 Clip 74915 (CIP)
Hafnia alvei 5 (AFSSA) Enterococcus faecalis 2 (AFSSA)
Klebsiella pneumoniae 8 ATCC 13883 Enterococcus faecium 1 (AFSSA)
Klebsiella terrigena 1 (CTSCCV) Bacillus cereus 4 (AFSSA)
Klebsiella oxytoca 3 (AFSSA) Bacillus subtilis 1 CIP52.65 (CIP)
Morganella. morganii 1 (AFSSA) Bacillus megaterium 1 (AFSSA)
Pantoea sp. 3 (AFSSA) Bacillus lentus 1 (AFSSA)
Pantoea agglomerans 1 ATCC 27155 Staphylococcus aureus 3 i.e. ATCC 700699
Providencia alcalifaciens 1 (AFSSA) Staphylococcus haemolyticus 1 CIP 81.56 (CIP)
Providencia stuartii 1 (AFSSA) Staphylococcus simulans 1 CIP 81.64 (CIP)
Proteus vulgaris 2 (AFSSA) Staphylococcus lentus 1 CIP 81.63 (CIP)
Proteus morganii 3 (AFSSA) Staphylococcus equorum 1 CIP 103502 (CIP)
Proteus mirabilis 2 (AFSSA) Staphylococcus capitis 1 CIP 81.53 (CIP)
Photorhabdus luminescens 1 TTO1 (CIP) Streptococcus dysgalactiae 1 CIP 102914 (CIP)
Shigella sonnei 2 (AFSSA) Streptococcus equinus 1 CIP 103232 (CIP)
Shigella flexneri 1 ATCC 12022 Streptococcus bovis 1 CIP 102302 (CIP)
  1. IFF : 22 isolates from infant formulae factories, kindly provided by A. Leclercq (Institut Pasteur, Paris, France); BM : bioMérieux (Craponne, France) kindly provided by I. Desforges; BgVV: Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin (Germany); CTSCCV: Centre Technique de la Salaison, de la Charcuterie et des Conserves de Viandes (Maisons Alfort, France); CIP : Culture Collection of Institut Pasteur (Paris, France); AFSSA : Agence Française de Sécurité Sanitaire des Aliments (Maisons-Alfort, France).