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Table 2 LSMean values with Standard errors of the MMP9 gene transcripts. Depicts the LSMean values with Standard errors of the MMP9 gene transcripts expressed by macrophages over time and measured by Real Time RT PCR as described in materials and methods. Values with same superscript (a, b, c, d) indicate significant differences among the strains at that time point PI.

From: Cytokine responses of bovine macrophages to diverse clinical Mycobacterium avium subspecies paratuberculosis strains

Strain

Time post infection

 

2 hour

16 hour

24 hour

48 hour

96 hour

B1018

3 ± 0.47

18.2 ± 0.81

82.7 ± 19.2

109.8 ± 12.3c

66.6 ± 10d

S7565

1.4 ± 0.09

0.07 ± 0.03

130.8 ± 8.3a

760.3 ± 26c

117.4 ± 6.3

Hu6

4.2 ± 0.72

10.9 ± 0.64

45.9 ± 2.25a,b

321.3 ± 29.8

629 ± 49.2

M. avium

0.94 ± 0.27

53.2 ± 1.3

131.3 ± 10.9b

318.5 ± 38.9

220.6 ± 9.7d

PC1x

0.7 ± 0.06

49.4 ± 3.1

648 ± 9.5

365.8 ± 8.9

19.8 ± 1.8

PC2y

0.44 ± 0.01

65 ± 2.2

294 ± 10

1564 ± 91.9

5.4 ± 2.2

NCz

0.3 ± 0.07

2.07 ± 0.2

5.6 ± 2.7

0.1 ± 0.01

6.7 ± 0.23

  1. x indicates positive control 1 (PC1) – cells stimulated with LPS
  2. y indicates positive control 2 (PC2) – cells stimulated with LPS in conjunction with recombinant bovine Interferon gamma
  3. z indicates negative control (NC) – unstimulated cells