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Table 2 LSMean values with Standard errors of the MMP9 gene transcripts. Depicts the LSMean values with Standard errors of the MMP9 gene transcripts expressed by macrophages over time and measured by Real Time RT PCR as described in materials and methods. Values with same superscript (a, b, c, d) indicate significant differences among the strains at that time point PI.

From: Cytokine responses of bovine macrophages to diverse clinical Mycobacterium avium subspecies paratuberculosis strains

Strain Time post infection
  2 hour 16 hour 24 hour 48 hour 96 hour
B1018 3 ± 0.47 18.2 ± 0.81 82.7 ± 19.2 109.8 ± 12.3c 66.6 ± 10d
S7565 1.4 ± 0.09 0.07 ± 0.03 130.8 ± 8.3a 760.3 ± 26c 117.4 ± 6.3
Hu6 4.2 ± 0.72 10.9 ± 0.64 45.9 ± 2.25a,b 321.3 ± 29.8 629 ± 49.2
M. avium 0.94 ± 0.27 53.2 ± 1.3 131.3 ± 10.9b 318.5 ± 38.9 220.6 ± 9.7d
PC1x 0.7 ± 0.06 49.4 ± 3.1 648 ± 9.5 365.8 ± 8.9 19.8 ± 1.8
PC2y 0.44 ± 0.01 65 ± 2.2 294 ± 10 1564 ± 91.9 5.4 ± 2.2
NCz 0.3 ± 0.07 2.07 ± 0.2 5.6 ± 2.7 0.1 ± 0.01 6.7 ± 0.23
  1. x indicates positive control 1 (PC1) – cells stimulated with LPS
  2. y indicates positive control 2 (PC2) – cells stimulated with LPS in conjunction with recombinant bovine Interferon gamma
  3. z indicates negative control (NC) – unstimulated cells