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Table 1 LSMean values with Standard errors of the MMP3 gene transcripts. Depicts the LSMean values with Standard errors of the MMP3 gene transcripts expressed by macrophages over time and measured by Real Time RT PCR as described in materials and methods. Values with same superscript (a, b, c, p, q, r) indicate significant differences among the strains at that time point PI.

From: Cytokine responses of bovine macrophages to diverse clinical Mycobacterium avium subspecies paratuberculosis strains

Strain

Time post infection

 

2 hour

16 hour

24 hour

48 hour

96 hour

B1018

44.3 ± 4.77

574.6 ± 315a

259.4 ± 50.5p

121.6 ± 26

72.3 ± 24.3

S7565

55.6 ± 5.17

25.7 ± 6.6b

10057 ± 2104p,q,r

5451 ± 453

406 ± 17.9

Hu6

5.4 ± 0.2

25 ± 3.9c

134 ± 19.6r

319 ± 6.2

61.2 ± 10.7

M. avium

22.3 ± 2.2

6794 ± 133a,b,c

846 ± 107q

166.7 ± 22.8

149 ± 3.6

PC1x

56.6 ± 8.5

555 ± 51.6

26180 ± 4595

16742 ± 2938

1840 ± 678

PC2y

188.8 ± 39

15825 ± 2625

8164 ± 160

35563 ± 3307

554 ± 8.1

NCz

14.8 ± 7.5

43.9 ± 3.6

398 ± 29.4

2629 ± 77.3

1.7 ± 0.18

  1. x indicates positive control 1 (PC1) – cells stimulated with LPS
  2. y indicates positive control 2 (PC2) – cells stimulated with LPS in conjunction with recombinant bovine Interferon gamma
  3. z indicates negative control (NC) – unstimulated cells