Figure 4

TNFα protein secreted by MDM cells exposed to MAP over time was measured by ELISA. Total amount of protein (pg/ml) is plotted on Y-axis (note that the Y1-axis scales have been optimized for magnitude of TNFα expression for each strain). Intracellular bacterial numbers were calculated based on the genome size of MAP and represented as genome equivalents (GE) on second Y-axis. MDMs stimulated with B1018 secreted low amounts of TNFα protein relative to other cell stimulations.