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Figure 3 | BMC Microbiology

Figure 3

From: Cytokine responses of bovine macrophages to diverse clinical Mycobacterium avium subspecies paratuberculosis strains

Figure 3

TNFα mRNA expressed by MDM cells exposed to MAP over time was measured by Real time RT PCR and fold change in gene expression relative to β actin was calculated by 2-ΔΔCT method. Mean fold change in gene expression is plotted on Y-axis (note that the Y1-axis scales have been optimized for magnitude of IL-10 expression for each strain). Intracellular bacterial numbers based on the amplification of hsp65 were calculated based on the genome size of MAP and represented as genome equivalents (GE) on second Y-axis. MDMs stimulated with B1018 expressed lower levels of TNFα mRNA relative to other cell stimulations.

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