Figure 2

Multiple alignment of the FHA domain from EscD with other FHA domains. Alignment is presented using default CHROMA [155] settings: Consensus abbreviations (amino acids): a, aromatic (FHWY, blue lettering on a dark yellow background); b, big (EFHIKLMQRWY, blue on light yellow); h, hydrophobic (ACFGHILMTVWY, black on dark yellow); l, aliphatic (ILV, grey on dark yellow); p, polar (CDEHKNQRST, blue on white); s, small (ACDGNPSTV, dark green on white); t, tiny (AGS, light green on white); -, negatively-charged (DE, red on white); and, +, positively-charged (KR, blue on white), c, charged (DEKRH, pink on white. Organism and gene name abbreviations used: EHEC (Escherichia coli O157:H7) EscD (ECs4558/dbj|BAB37981), Vp (Vibrio parahaemolyticus) PscD(ref|NP_798074), Pa(Pseudomonas aeruginosa) PscD (ref|NP_250408), Ah (Aeromonas hydrophila) AscD(gb|AAS91829), Pl (Photorhabdus luminescens) LscD(gb|AAO18032), Yp (Yersinia enterocolitica) YscD(gb|AAC37021), Dv (Desulfovibrio vulgaris) YscD(ref|YP_009153), Ct (Chlamydia trachomatis) CT664 (NP_220183), Cp (Chlamydophila pneumoniae) CpB0739(gb|AAP98668), Xa (Xanthomonas axonopodis) HrpD5 (gb|BAD29996), Ea (Erwinia amylovora) HrpQ(gb|AAB06000). The remaining 12 sequences are representative members of the SMART FHA domain (SM00240). Gene names, numbering and alignment are as presented in the SMART FHA family alignment http://www.smart.ox.ac.uk/.