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Figure 4 | BMC Microbiology

Figure 4

From: Proteolytic cleavage of pertussis toxin S1 subunit is not essential for its activity in mammalian cells

Figure 4

Localization of the processing site on S1. (A) CHO cells were incubated with PT or PT*-CSP/N (PT*/N) for 4 h and analyzed for S1 processing as before. Western blots are shown of the processing samples (proc) or purified protein loading controls (C). (B) Western blot comparing S1p after processing of PT in CHO cells (proc) or trypsin cleavage (tryp) of PT (200 or 100 ng).

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