Figure 2
From: Specific and sensitive detection of the conifer pathogen Gremmeniella abietina by nested PCR
Specificity test of different PCR assays. A, amplification using fungal universal primer pair NS1/8; B and C, amplification using specific primer pairs NS.Grem3/4 and NS.Grem5/6, respectively. G. abietina isolates 1–19 were not shown on this gel due to space limitation. Nested PCR using NS.Grem3/4 and NS.Grem5/6 as inner primers gave amplification patterns identical to B and C, respectively. Fungal strains in each lane are in the order given in Table 1 and 2. M: 1 kb Plus DNA Ladder (Invitrogen).