M protein typing of Thai group A streptococcal isolates by PCR-Restriction fragment length polymorphism analysis

BMC Microbiology

Table 1 PCR products and Alu I restriction fragment sizes and distribution in the Thai GAS isolates studied.

M type	Size of PCR product (bp)	Size of restriction fragment (bp)	No. (%) of isolates from PCR-RFLP analysis	M type	Size of PCR product (bp)	Size of restriction fragment (bp)	No. (%) of isolates from PCR-RFLP analysis
M1	1071	354	16 (15.09%)	M49	450	336	2 (1.89%)
	892	257				252
	744	218				196
	592	147				135
		110				89
		87		Emm 58.5	545	370	1 (0.94%)
		47				185
		37		M66	566	252	1 (0.94%)
		29			450	206
M4	747	314	2 (1.89%)			148
	609	174				123
	476	57		M81	649	322	7 (6.60%)
		47			493	185
M11	710	593	8 (7.55%)		375	127
	592	434				84
	450	147				62
		117		M89	404	350	1 (0.94%)
M12	1121	349	3 (2.83%)			77
		116		M93	679	254	22 (20.75%)
		93			540	227
		78			411	168
		59				130
		51				93
M25	649	247	10 (9.43%)			53
	540	203		M102	420	332	1 (0.94%)
		148				92
		51		M104	450	210	3 (2.83%)
		37				146
M33	812	347	3 (2.83%)			118
	670	168		M109	509	386	3 (2.83%)
	541	94				168
		74		M112	450	435	5 (4.72%)
M44/61	450	212	11 (10.38%)			104
		133		st11014	744	596	5 (4.72%)
M48	710	162	2 (1.89%)		620	87
	592	121			493	29
	471	81

ISSN: 1471-2180