Table 1 Primers used in gene inactivation and confirmation
From: ABC transporter FtsABCD of Streptococcus pyogenes mediates uptake of ferric ferrichrome
Primer | Sequence (5'-3') | Purpose |
|---|---|---|
1 | ACCATGGCTATGCCTCAAATTGCTGG | ftsB inactivation |
2 | ACCATGGTTTTCCAATCTTTTAACCAC | |
3 | ACCATGGAGCTTATGTGTTGCTATTTAC | ftsC inactivation |
4 | ACCATGGATAGCTCCTGCTAAGACAAG | |
5 | ACCATGGTCTTTTAGTAGCTTGTTCGTC | mtsA inactivation |
6 | ACCATGGTTCATTTCGACAAGAGGCTG | |
7 | AACTCTACTATTAACACTCTG | confirmation of ftsB inactivation |
8 | GCAACGACGACATAATCACC | |
9 | GTGTCTCGAGCGTTTTTGC | confirmation of ftsC inactivation |
10 | CAATATAGCCGATCATTTG | |
11 | ATGAGCCTCATTTTGGGTGC | confirmation of mtsA inactivation |
12 | GCCACATAAGCTTTTAGGTTC | |
13 | AGAATTTTGTTAGCAGTTCG | aad-specific, paired with primer 7, 9, or 11 for inactivation confirmation |
14 | CTTTGAGTGAGCTGATACCG | pFWaad-specific, paired with primer 8, 10, or 12 for inactivation confirmation |