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Figure 1 | BMC Microbiology

Figure 1

From: Genometrics as an essential tool for the assembly of whole genome sequences: the example of the chromosome of Bifidobacterium longum NCC2705

Figure 1

Genometric analyses of the two chromosomal configurations of B. longum NCC2705. Cumulative GC- and ORF orientation skew analyses of chromosomal configurations I (A, B) and II (C) of B. longum NCC2705. Configuration I [GenBank:NC_004307.1] is plotted without modification with respect to the version available on databases (Watson strand), (A) as well as the Crick strand of same sequence starting with the gene dnaA (B). Cumulative GC skew of the first codon position (black curve) and cumulative ORF orientation skew (grey curve) are plotted as a function of their position on the genome. Distance between two graduation marks represents an excess of 104 Gs over Cs in the first codon position cumulative GC skew and of 2·103 nucleotides in the ORF orientation skew. Thin arrows indicate relevant changes of the slope sign. The thick arrow corresponds to the position and the orientation of dnaA. The location of the integrated plasmid described in [4] is indicated. Vertical lines specify the position of copies of ISBlo2 (dashed line) and ISBlo5 (full line) insertion sequences. Segments delimited by these insertion sequences are designated a, b, c and d. Horizontal lines below the curves are the hits of scaffolds 1 (black), 8 (light grey) and 9 (dark grey) of B. longum DJO10A when BLASTed against the B. longum NCC2705 genome in configurations I (A, B) or II (C).

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