Skip to main content

Advertisement

Table 1 Proteins showing differential expression on 2-D gels.

From: Polyamine stress at high pH in Escherichia coli K-12

   Differential expression1  
Spot no. Protein Spermine (1 mM) Spermidine (10 mM) pH 8.5 Known or predicted function
   pH 7.0 pH 8.5   
1 PykF 0.34 ± 0.09 (+) (+) Pyruvate kinase
2 PykF 0.39 ± 0.06 (+) (+) Pyruvate kinase
3 GlpK 0.68 ± 0.09 0.63 ± 0.12 0.50 ± 0.09 Glycerol kinase
4 SerS 0.83± 0.08 0.60 ± 0.1 0.60 ± 0.1 Serine-tRNA ligase
5 ThrC -0.34 ± 0.06    Threonine synthase
6   0.51 ± 0.03 0.81 ± 0.07   
7 TnaA   0.92 ± 0.06 0.87 ± 0.09 Tryptophanase
8 TnaA   0.53 ± 0.07 0.77 ± 0.07 Tryptophanase
9 MalE -1.04 ± 0.05    Maltose-binding, periplasmic
10 MalE -1.0 ± 0.00    Maltose-binding, periplasmic
11 MalE     Maltose-binding, periplasmic
12 MalE -0.74 ± 0.08    Maltose-binding, periplasmic
13 Asd -0.54 ± 0.10   -0.42 ± 0.07 Aspartate semialdehyde dehydrogenase
14 FabB (-) (-) -0.64 ± 0.11 Beta-Ketoacyl-ACP synthase I
15 DeaD 0.27 ± 0.03 0.41 ± 0.08   ATP-dependent RNA helicase
16 TktA -0.46 ± 0.11 (-) -0.48 ± 0.07 Transketolase
17 OmpC (+) 0.56 ± 0.05   Outer membrane protein C
18 OmpF 0.96 ± 0.03 0.74 ± 0.15   Outer membrane porin
19 OmpF 0.56 ± 0.05 0.90 ± 0.08   Outer membrane porin
20 Pta (-) -0.50 ± 0.15 (-) Phosphate aceyltransferase
21   -0.47 ± 0.06 (-) (-)  
22 MglB    0.23 ± 0.02 Galactose-binding protein
23   0.69 ± 0.11 (+)   
24 MalM -59 ± 0.10    Maltose periplasmic protein
25 SucB -0.25 ± 0.02 -0.89 ± 0.08   Dihydrolipoamide Succinyltransferase
26 GlnS (-) -0.59 ± 0.11 -0.60 ± 0.1 Glutaminyl-tRNA synthetase
27 NanA   0.49 ± 0.12   Putative N-acetylmanosamine-6-phosphate 2-epimerase
28 Lpd (-) -0.79 ± 0.1 -0.69 ± 0.1 Dihydrolipoamide dehydrogenase
29 Lpd (-) -0.55 ± 0.1 -0.72 ± 0.17 Dihydrolipoamide dehydrogenase
30 Lpd (-) -0.58 ± 0.1 -0.27 ± 0.05 Dihydrolipoamide dehydrogenase
31 FabE   -0.24 ± 0.04 -0.82 ± 0.07 Acetyl-CoA carboxylase
32 RpsB (-) -0.26 ± 0.05   30S ribosomal subunit protein S2
33 YrdA   0.26 ± 0.04 0.44 ± 0.12  
34 OmpA   0.27 ± 0.04 0.43 ± 0.09 Outer membrane protein A
35 RpsF -0.22 ± 0.01 -0.85 ± 0.09 -0.50 ± 0.12 30S ribosomal subunit protein S6
36 RpsF -0.22 ± 0.01    30S ribosomal subunit protein S6
37 Gpt 0.18 ± 0.03 0.40 ± 0.08 0.58 ± 0.1  
38 TpiA -0.28 ± 0.03 -0.57 ± 0.06 (-) Triosephosphate isomerase
39 RapA (HepA) -0.61 ± 0.02 -0.76 ± 0.09 -0.63 ± 0.11 RNA polymerase binding protein
40 YfiD -0.27 ± 0.02 -0.21± 0.02 -0.37 ± 0.03 Pyruvate formate-lyase homolog
41 GapA -0.51 ± 0.1 -0.87 ± 0.06 -0.50 ± 0.13 Glyceraldehyde 3-phosphate dehydrogenase A
42 AtpA -0.27 ± 0.07    ATP synthase subunit alpha
43   -0.74 ± 0.02 (-) (-)  
44 GuaB    -0.75 ± 0.1 Inosine-5'-monophosphate dehydrogenase
  1. 1Relative differential expression of protein, shown as LDE ± standard error (n = 9), was determined as described under Materials and Methods. Symbols: (+), induced; (-) repressed, representing spots that showed differential expression based on visual inspection, although not quantifiable by the Z3 software.