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Table 1 Proteins showing differential expression on 2-D gels.

From: Polyamine stress at high pH in Escherichia coli K-12

  

Differential expression1

 

Spot no.

Protein

Spermine (1 mM)

Spermidine (10 mM) pH 8.5

Known or predicted function

  

pH 7.0

pH 8.5

  

1

PykF

0.34 ± 0.09

(+)

(+)

Pyruvate kinase

2

PykF

0.39 ± 0.06

(+)

(+)

Pyruvate kinase

3

GlpK

0.68 ± 0.09

0.63 ± 0.12

0.50 ± 0.09

Glycerol kinase

4

SerS

0.83± 0.08

0.60 ± 0.1

0.60 ± 0.1

Serine-tRNA ligase

5

ThrC

-0.34 ± 0.06

  

Threonine synthase

6

 

0.51 ± 0.03

0.81 ± 0.07

  

7

TnaA

 

0.92 ± 0.06

0.87 ± 0.09

Tryptophanase

8

TnaA

 

0.53 ± 0.07

0.77 ± 0.07

Tryptophanase

9

MalE

-1.04 ± 0.05

  

Maltose-binding, periplasmic

10

MalE

-1.0 ± 0.00

  

Maltose-binding, periplasmic

11

MalE

   

Maltose-binding, periplasmic

12

MalE

-0.74 ± 0.08

  

Maltose-binding, periplasmic

13

Asd

-0.54 ± 0.10

 

-0.42 ± 0.07

Aspartate semialdehyde dehydrogenase

14

FabB

(-)

(-)

-0.64 ± 0.11

Beta-Ketoacyl-ACP synthase I

15

DeaD

0.27 ± 0.03

0.41 ± 0.08

 

ATP-dependent RNA helicase

16

TktA

-0.46 ± 0.11

(-)

-0.48 ± 0.07

Transketolase

17

OmpC

(+)

0.56 ± 0.05

 

Outer membrane protein C

18

OmpF

0.96 ± 0.03

0.74 ± 0.15

 

Outer membrane porin

19

OmpF

0.56 ± 0.05

0.90 ± 0.08

 

Outer membrane porin

20

Pta

(-)

-0.50 ± 0.15

(-)

Phosphate aceyltransferase

21

 

-0.47 ± 0.06

(-)

(-)

 

22

MglB

  

0.23 ± 0.02

Galactose-binding protein

23

 

0.69 ± 0.11

(+)

  

24

MalM

-59 ± 0.10

  

Maltose periplasmic protein

25

SucB

-0.25 ± 0.02

-0.89 ± 0.08

 

Dihydrolipoamide Succinyltransferase

26

GlnS

(-)

-0.59 ± 0.11

-0.60 ± 0.1

Glutaminyl-tRNA synthetase

27

NanA

 

0.49 ± 0.12

 

Putative N-acetylmanosamine-6-phosphate 2-epimerase

28

Lpd

(-)

-0.79 ± 0.1

-0.69 ± 0.1

Dihydrolipoamide dehydrogenase

29

Lpd

(-)

-0.55 ± 0.1

-0.72 ± 0.17

Dihydrolipoamide dehydrogenase

30

Lpd

(-)

-0.58 ± 0.1

-0.27 ± 0.05

Dihydrolipoamide dehydrogenase

31

FabE

 

-0.24 ± 0.04

-0.82 ± 0.07

Acetyl-CoA carboxylase

32

RpsB

(-)

-0.26 ± 0.05

 

30S ribosomal subunit protein S2

33

YrdA

 

0.26 ± 0.04

0.44 ± 0.12

 

34

OmpA

 

0.27 ± 0.04

0.43 ± 0.09

Outer membrane protein A

35

RpsF

-0.22 ± 0.01

-0.85 ± 0.09

-0.50 ± 0.12

30S ribosomal subunit protein S6

36

RpsF

-0.22 ± 0.01

  

30S ribosomal subunit protein S6

37

Gpt

0.18 ± 0.03

0.40 ± 0.08

0.58 ± 0.1

 

38

TpiA

-0.28 ± 0.03

-0.57 ± 0.06

(-)

Triosephosphate isomerase

39

RapA (HepA)

-0.61 ± 0.02

-0.76 ± 0.09

-0.63 ± 0.11

RNA polymerase binding protein

40

YfiD

-0.27 ± 0.02

-0.21± 0.02

-0.37 ± 0.03

Pyruvate formate-lyase homolog

41

GapA

-0.51 ± 0.1

-0.87 ± 0.06

-0.50 ± 0.13

Glyceraldehyde 3-phosphate dehydrogenase A

42

AtpA

-0.27 ± 0.07

  

ATP synthase subunit alpha

43

 

-0.74 ± 0.02

(-)

(-)

 

44

GuaB

  

-0.75 ± 0.1

Inosine-5'-monophosphate dehydrogenase

  1. 1Relative differential expression of protein, shown as LDE ± standard error (n = 9), was determined as described under Materials and Methods. Symbols: (+), induced; (-) repressed, representing spots that showed differential expression based on visual inspection, although not quantifiable by the Z3 software.