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Table 4 Bacterial strains and plasmids used or created in this study

From: The two authentic methionine aminopeptidase genes are differentially expressed in Bacillus subtilis

Strains

Genotype

Source or Reference

Escherichia coli

XL1-Blue

K12 supE 44 hsdR 17 recA 1 endA 1 gyrA 46 thi relA 1 lac- F'[proAB+ lacIq lacZΔM 15 Tn10(tetR)]

Laboratory collection

TG1

K12 supE hsdΔ 5 thi Δ (lac-proAB) F'[traD 36 proA+ proB+ lacIq lacZ ΔM 15]

Laboratory collection

BL5(DE3)

F-

Invitrogen

EM9

PT7-map (on chromosome)

Chiron Culture Collection Centre

ECIP8003

F- pIPP8003

This work

ECIP8004

F- pIPP8004

This work

ECHP1005

EM9 pHPP1017

This work

ECHP1006

EM9 pHPP1018

This work

ECHP1007

EM9 pBAD

This work

Bacillus subtilis

168

trp C2

Laboratory collection

BFS4611

trp C2 yflG::lacZ

Functional analysis projecta

BFS817

trp C2 yvoA::lacZ

Functional analysis projecta

BSHP7042

trp C2 map-lacZ

This work

BSHP7045

trp C2 pDG148-map

This work

BSHP7046

trp C2 map::lacZ pDG148-map

This work

BSHP7037

trp C2 map::lacZ pDG148-yflG

This work

BSHP7038

trp C2 pDG148-yflG

This work

BSHP7043

trp C2 yflH::lacZ

This work

BSIP8001

trp C2 amyE::gfp-map

This work

BSIP8002

trp C2 amyE::gfp-yflG

This work

BSIP8003

trp C2 yvoA::[(lacZ-ery)::pEC23(Kan)]

This work

BSIP8004

trp C2 yflG::lacZ yvoA::[(lacZ-ery)::pEC23(Kan)]

This work

Plasmids

Description

Source or Reference

pET24b(+)

expression vector, KanR

Novagen

pBAD-abg

genetic rescue vector, AmpR

[43]

pDG148

expression vector, AmpR KanR

[44]

pJM783

cloning vector, CmR

[45]

pSG1729

N-terminal GFP fusion vector, AmpR SpecR

[42]

pEC23

integrative plasmid, KanR

M. Simon and P. Stragier, unpublished

pIPP8003

pET24b map

This work

pIPP8004

pET24b yflG

This work

pHPP1017

pBAD map

This work

pHPP1018

pBAD yflG

This work

pHPP7017

pJM783 map-lacZ

This work

pHPP7018

pJM783 yflH::lacZ

This work

pHPP7021

pDG148 map

This work

pHPP7022

pDG148 yflG

This work

pHPP7025

pJM783 map::lacZ

This work

pIPP8001

pSG1729 map

This work

pIPP8002

pSG1729 yflG

This work

  1. a This strain has been constructed during the frame of the project for the functional characterization of the genome of B. subtilis in Japan [46].