From: The two authentic methionine aminopeptidase genes are differentially expressed in Bacillus subtilis
Strains | Genotype | Source or Reference |
---|---|---|
Escherichia coli | ||
XL1-Blue | K12 supE 44 hsdR 17 recA 1 endA 1 gyrA 46 thi relA 1 lac- F'[proAB+ lacIq lacZΔM 15 Tn10(tetR)] | Laboratory collection |
TG1 | K12 supE hsdΔ 5 thi Δ (lac-proAB) F'[traD 36 proA+ proB+ lacIq lacZ ΔM 15] | Laboratory collection |
BL5(DE3) | F- | Invitrogen |
EM9 | PT7-map (on chromosome) | Chiron Culture Collection Centre |
ECIP8003 | F- pIPP8003 | This work |
ECIP8004 | F- pIPP8004 | This work |
ECHP1005 | EM9 pHPP1017 | This work |
ECHP1006 | EM9 pHPP1018 | This work |
ECHP1007 | EM9 pBAD | This work |
Bacillus subtilis | ||
168 | trp C2 | Laboratory collection |
BFS4611 | trp C2 yflG::lacZ | Functional analysis projecta |
BFS817 | trp C2 yvoA::lacZ | Functional analysis projecta |
BSHP7042 | trp C2 map-lacZ | This work |
BSHP7045 | trp C2 pDG148-map | This work |
BSHP7046 | trp C2 map::lacZ pDG148-map | This work |
BSHP7037 | trp C2 map::lacZ pDG148-yflG | This work |
BSHP7038 | trp C2 pDG148-yflG | This work |
BSHP7043 | trp C2 yflH::lacZ | This work |
BSIP8001 | trp C2 amyE::gfp-map | This work |
BSIP8002 | trp C2 amyE::gfp-yflG | This work |
BSIP8003 | trp C2 yvoA::[(lacZ-ery)::pEC23(Kan)] | This work |
BSIP8004 | trp C2 yflG::lacZ yvoA::[(lacZ-ery)::pEC23(Kan)] | This work |
Plasmids | Description | Source or Reference |
pET24b(+) | expression vector, KanR | Novagen |
pBAD-abg | genetic rescue vector, AmpR | [43] |
pDG148 | expression vector, AmpR KanR | [44] |
pJM783 | cloning vector, CmR | [45] |
pSG1729 | N-terminal GFP fusion vector, AmpR SpecR | [42] |
pEC23 | integrative plasmid, KanR | M. Simon and P. Stragier, unpublished |
pIPP8003 | pET24b map | This work |
pIPP8004 | pET24b yflG | This work |
pHPP1017 | pBAD map | This work |
pHPP1018 | pBAD yflG | This work |
pHPP7017 | pJM783 map-lacZ | This work |
pHPP7018 | pJM783 yflH::lacZ | This work |
pHPP7021 | pDG148 map | This work |
pHPP7022 | pDG148 yflG | This work |
pHPP7025 | pJM783 map::lacZ | This work |
pIPP8001 | pSG1729 map | This work |
pIPP8002 | pSG1729 yflG | This work |