Sequences alignment between MAP from E. coli , B. subtilis , and S. aureus . The first line shows the protein sequence of MAP from B. subtilis (MAP_Bs), the second shows MAP from E. coli (MAP_Ec), the third shows YflG from B. subtilis (YflG_Bs) and the last shows YflG/MAP from Staphylococcus aureus (YMAP_Sa). Identical amino acids are shadowed in dark and conservative amino acids are shadowed in grey. The highlighted residues stand for those conserved amino acids important for MAP activity (bold and italic residues are conservative basic ones, while bold only are acidic ones). [MFY]-x-G-H-G-[LIVMC]-[GSH]-x(3)-H-x(4)-[LIVM]-x-[HN]-[YWVH] (where H is a cobalt ligand) is the conserved motif within the protein sequences (placed under the alignment).