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Figure 1 | BMC Microbiology

Figure 1

From: A gonococcal homologue of meningococcal γ-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent

Figure 1

The presence of a meningococcal ggt gene homologue in N. gonorrhoeae . A. A schematic diagram showing the position of the set of primers used for the detection of ggt gene homologues. The black bar shows the region of the DNA probe used for Southern blotting in panel C. B. Amplification of gonococcal ggh gene by PCR. The genomic DNAs of neisserial species used for PCR were as follows: lane 1, H44/76 (N. meningitidis ggt+); lane 2, NIID113 (N. meningitidis ggt:: IS) [49]; lane 3–13, ATCC49226, NIID54, NIID102, NIID103, NIID104, NIID105, NIID106, NIID107, NIID108, NIID109, NIID111 (N. gonorrhoeae). C. Southern blotting using the meningococcal ggt gene as a probe. Two micrograms of purified chromosomal DNA digested with Cla I were subjected to this analysis. Lane 1, H44/76 (N. meningitidis ggt+); lane 2, NIID113 (N. meningitidis ggt:: IS) [49]; lane 3, NIID54 (N. gonorrhoeae); lane 4, ATCC23970 (N. lactamica); lane 5, ATCC13120 (N. flavescens); lane 6, ATCC14686 (N. denitrificans); lane 7, ATCC25295 (N. elongata); lane 8, ATCC14687 (N. canis); lane 9, ATCC14685 (N. cinerea); lane 10, NIID16 (N. mucosa); lane 11, NIID17 (N. sicca).

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