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Table 3 Susceptibility of Escherichia coli JM109 to metal cations with 2 or 24 h of exposure in minimal (MSVG) medium

From: High-throughput metal susceptibility testing of microbial biofilms

Periodic group Metal ion Exposure time (h) MIC (mM) MBC (mM) MBEC (mM) Fold Tolerance1
7B Mn2+ 2 33 ± 9 > 149 > 149 na
   24   93 ± 37 84 ± 47 0.90
8B Co2+ 2 ≤ 0.27 26 ± 10 > 139 ≥ 11
   24   1.0 ± 3 1.0 ± 3 1.0
  Ni2+ 2 ≤ 0.27 > 139 > 139 na
   24   0.48 ± 0.13 0.54 ± 0.39 1.1
1B Cu2+ 2 ≤ 0.25 ≤ 0.25 ≤ 0.25 na
   24   ≤ 0.25 ≤ 0.25 na
  Ag+ 2 ≤ 0.04 ≤ 0.04 0.59 ± 0 ≥ 15
   24   ≤ 0.04 ≤ 0.04 1.0
2B Zn2+ 2 ≤ 0.25 11 ± 6 > 125 ≥ 23
   24   0.55 ± 0.31 0.49 ± 0.35 0.89
  Cd2+ 2 ≤ 0.14 5.1 ± 2.9 > 73 ≥ 29
   24   0.49 ± 0.14 1.4 ± 1.1 2.9
  Hg2+ 2 ≤ 0.02 ≤ 0.02 0.05 ± 0.02 ≥ 2.5
   24   ≤ 0.02 ≤ 0.02 1.0
3A Al3+ 2 nd 42 ± 40 > 304 ≥ 14
   24   1.5 ± 0.6 4.0 ± 1.4 2.7
4A Sn2+ 2 2.2 ± 0 8.6 ± 0 8.6 ± 0 1.0
   24   4.3 ± 0 7.5 ± 2.2 1.7
  Pb2+ 2 nd 20 ± 0 40 ± 0 2.0
   24   4.9 ± 0 1.2 ± 0 0.25
  1. na indicates a measurement that is not applicable
  2. bold indicates the fold tolerance at 24 h of exposure
  3. 1the fold tolerance, given the sensitivity of the assay on a log2 scale, is equal to the ratio of the means of MBEC:MBC100