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Table 2 Susceptibility of Escherichia coli JM109 to metal cations with 2 or 24 h of exposure in rich (LB + B1) medium

From: High-throughput metal susceptibility testing of microbial biofilms

Periodic group Metal ion Exposure time (h) MIC (mM) MBC (mM) MBEC (mM) Fold Tolerance1
7B Mn2+ 2 37 ± 0 > 149 > 149 na
   24   198 ± 86 223 ± 86 1.1
8B Ni2+ 2 7.5 ± 2.1 > 140 >140 na
   24   17 ± 0 29 ± 11 1.7
1B Cu2+ 2 4.5 ± 1.4 16 ± 0 16 ± 0 1.0
   24   16 ± 0 16 ± 0 1.0
  Ag+ 2 0.06 ± 0.02 0.06 ± 0.02 1.6 ± 0.7 24
   24   0.06 ± 0.02 0.04 ± 0 0.7
2B Zn2+ 2 4.2 ± 2.4 125 ± 0 > 125 ≥ 2.0
   24   31 ± 0 31 ± 0 1.0
  Cd2+ 2 1.1 ± 0.2 55 ± 21 73 ± 0 1.3
   24   2.3 ± 0 3.0 ± 1.3 1.3
  Hg2+ 2 0.04 ± 0.02 0.14 ± 0.04 0.32 ± 0.22 2.3
   24   0.04 ± 0 0.04 ± 0 1.0
3A Al3+ 2 nd > 304 > 304 na
   24   19 ± 0 19 ± 0 1.0
4A Sn2+ 2 nd > 17 > 17 na
   24   17 ± 0 17 ± 0 1.0
  1. A portion of the data in this table (at the 24 h timepoint) represents a greater number of trials and reanalysis of the data originally reported in an earlier study [10].
  2. na indicates a calculation that is not applicable
  3. nd indicates an MIC that could not be determined due to metal precipitation
  4. bold indicates the fold tolerance at 24 h of exposure
  5. 1the fold tolerance, given the sensitivity of the assay on a log2 scale, is equal to the ratio of the means of MBEC:MBC100