Table 2 Summary of preparation methods A-G

A

Sample heated for 15 min in Nutrient Broth at 65°C, then plated on Sporulation plates with 20 IU/ml penicillin G

B

4 hour Pre-incubation of sample in L-broth (30°C shaking at 250 rpm) then heated for 15 min at 65°C then plated on Sporulation plates

C

Same as Method B but plated on Sporulation plates with 20 IU/ml penicillin G

D

4 hour Pre-incubation of sample in L-broth/acetate [26] (30°C shaking at 250 rpm) then heated for 15 min at 65°C then plated on Sporulation plates

E

Same as Method D above except plated on Sporulation plates with 20 IU/ml penicillin G

F

Sample heated for 15 min in L-broth at 65°C then plated on Sporulation plates

G

Same as Method F above except plated on Sporulation plates with 20 IU/ml penicillin G