BMC Microbiology

Table 1 Isolates positive for cry and cyt genes as determined by Probe Hybridization and PCR.

From: A Bacillus thuringiensis isolation method utilizing a novel stain, low selection and high throughput produced atypical results

Sample number	Isolate #	cry1	cry1 C	cry1 D	cry2	cry 3,7,8	cry4	cry5/Nem	cyt1	cyt2
22	Bt1-33							H-P
22	Bt1-35							H
72	Bt2-46	H-P			H-P
81	Bt2-48				P
N/A	Bt2-50	H-P	P	P	H-P
N/A	Bt2-51	H			H-P					H
N/A	Bt2-52	H-P	P	P	H-P
N/A	Bt2-53						H-P			***
N/A	Bt2-54						H-P		H-P	H
N/A	Bt2-55					H-P				***
40	Bt1-65	P					H-P
58	Bt1-74						H
63	Bt1-83	H
11	Bt1-1						P
11	Bt1-4						P
11	Bt1-5						P
15	Bt1-6						P
41	Bt2-14						P			P
41	Bt2-15						H-P
71	Bt2-21									H
77	Bt2-24								P
82	Bt2-35								P

(H) denotes probe hybridization and (P) PCR. Samples Bt2-(50–55) were control strains where bold type denotes genes that strains are reported to contain, and (***) designates genes that are reported to be present but were not detected.

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ISSN: 1471-2180

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