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Table 1 Bacterial strains and plasmids used

From: The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803

Strain Description Reference/Source
Synechocystis 6803 Glucose-tolerant 'wild-type' strain [35]
pta ORF slr2132 inactivated by insertion of EmR gene into Pst I site at position 1,251,313 of the genome (872 bp into the coding seq of slr2132). This study
dspA ORF sll0698 inactivated by insertion of KmR gene into Eco R1site at position 125,060 of the genome (1384 bp into the coding seq of sll0698). This study
ptadspA ORF sll0698 inactivated in pta This study
pBluescriptII KS + Multipurpose cloning vector with AmpR. Stratagene
pBSEmEco1 Cloned EmR gene cassette. [48,49]
pUC4K Cloned KmR gene cassette Stratagene