Table 1 Detailed analysis of the effects of preincubating cultures of A. awamori with 3,5-DCP, Cr6+ and Zn2+ on [Ca2+]c response to 5 mM external CaCl2
From: A new short-term toxicity assay using Aspergillus awamori with recombinant aequorin gene
Toxicant | Features of [Ca2+]C Signatures | ||||||
|---|---|---|---|---|---|---|---|
A | LT50 | FRL (%) 5 min pre-incubation | RT (%) 5 min pre-incubation | FRL (%) 30 min pre-incubation | RT (%) 30 min pre-incubation | ||
3,5-DCP | 0.112 | ↑ | - | 111 ± 8.4 (↑) | 111 ± 8.4 (↑) | 106 ± 7.6 (-) | 98 ± 1.3 (-) |
(mgl-1) | 11.2 | ↓ | - | 120 ± 4.8 (↑) | 125 ± 4.6 (↑) | 127 ± 29.7 (-) | 134 ± 31.9 (↑) |
112 | ↓ | ↑ | 289 ± 17.6 (↑) | 292 ± 6.6 (↑) | 416 ± 23.2 (↑) | 416 ± 16.6 (↑) | |
Cr6+ | 15 | ↓ | - | - | - | - | - |
(mgl-1) | 120 | ↓ | - | - | - | - | - |
260 | ↓ | - | - | - | - | - | |
Zn2+ | 180 | ↑ | - | 106 ± 9.2(-) | 127 ± 4.6 (↑) | 97 ± 6.9(-) | 92 ± 1.9(↓) |
(mgl-1) | 350 | ↓ | - | 153 ± 13.6(↑) | 167 ± 14.4(↑) | 149 ± 11.4(↑) | 143 ± 10.2(↑) |
700 | ↓ | ↑ | 164 ± 30.7(↑) | 179 ± 26.9(↑) | 245 ± 16.5(↑) | 261 ± 9.6(↑) | |
1300 | ↓ | ↑ | 169 ± 14.8(↑) | 182 ± 10.4(↑) | 169± 12.6(↑) | 176 ± 16.5(↑) | |