Figure 4From: Genetical and functional investigation of fliC genes encoding flagellar serotype H4 in wildtype strains of Escherichia coli and in a laboratory E. coli K-12 strain expressing flagellar antigen type H48(A) Low power micrograph of E. coli strain TPE1978 cells showing the density of the bacterial samples used for indirect IEM and the presentation of flagella (bar length = 1 μm) (B) IEM of strain TPE1978 flagella after incubation with rabbit flagellar H48 antiserum (1:1000) and detection of bound antibody by anti-rabbit-IgG- 10 nm gold (1:20), bar length = 100 nm. (C) Strain JM109 flagella after incubation with rabbit flagellar H48 antiserum and detection of bound antibody by anti-rabbit-IgG- 10 nm gold. (D) Strain TPE1978 flagella after incubation with rabbit flagellar H4 antiserum (1:1000) and detection of bound antibody by anti-rabbit-IgG- 5 nm gold. (E) Strain JM109 flagella after incubation with rabbit flagellar H4 antiserum and detection of bound antibody by anti-rabbit-IgG- 5 nm gold. (F) Double-labeling IEM of strain TPE1978 after sequential incubations with rabbit flagellar H4 antiserum and anti-rabbit-IgG 5 nm gold, followed by rabbit flagellar H48 antiserum detected by anti-rabbit-IgG- 10 nm gold. Both, 5 nm and 10 nm gold markers are bound at comparable amounts over all flagella present on the bacteria. (G) Double-labeling IEM of strain JM109 after sequential incubations with rabbit flagellar H4 antiserum and anti-rabbit-IgG- 5 nm gold followed by rabbit flagellar H48 antiserum and anti-rabbit-IgG- 10 nm gold. Only H48 specific (10 nm) gold particles are bound to the flagella of JM109.Back to article page