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Figure 1 | BMC Microbiology

Figure 1

From: Immunization of mice with YscF provides protection from Yersinia pestis infections

Figure 1

Purified HT-YscF was run on SDS-PAGE gels (12.5% for Panels A and B, 15% Panel C) to analyze purity of the recovered protein. Panel A, separated proteins were stained with GelCode Blue stain (Pierce Chemical). Panel B, purified HT-YscF was immunoblotted to PVDF and probed with antibody specific for penta-histidine to identify which bands contained the His-tag. Panel C, purified HT-YscF was immunoblotted to PVDF and probed with post-immune serum from immunized mice.

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