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Table 2 Comparison of diverse culture variables for productivity of PRSP cells

From: Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniae

Variable

Phase 0

Phase 1

Phase 2

Cryoprotection Media

SM > 17%G:TSB

NA

NA

Inoculum (colonies)

NA

10 > 5 or 15 or loopful

NA

Culture Broth

NA

THB > BHI

THB > BHI

Yeast Extract (% YE)

0.5 > 0

2.0 > 0

2.0 > 0

Horse Blood (% HB)

NA

2.5 > 0

2.5 > 0

pH adjustment to 7.8

NA

Initially > NoAdj

q1h > NoAdj

Incubation time (h)

15 > 18 > 24

12 > 15, 18, 24

3*, 5†, 6‡

Ideal dilution (Tube #)

NA

5 > 4 > 1–3

6 = 7 = 8

  1. SM = Skim Milk; > implies "superior to"; NA = non applicable to that Phase; 17%G:TSB = 17% glycerol in trypticase soy broth; THB = Todd Hewitt Broth; BHI = Brain Heart Infusion; NoAdj= no adjustment; *Tube 6; †Tube 7; ‡Tube 8.