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Table 2 Comparison of diverse culture variables for productivity of PRSP cells

From: Optimization of culture conditions to obtain maximal growth of penicillin-resistant Streptococcus pneumoniae

Variable Phase 0 Phase 1 Phase 2
Cryoprotection Media SM > 17%G:TSB NA NA
Inoculum (colonies) NA 10 > 5 or 15 or loopful NA
Culture Broth NA THB > BHI THB > BHI
Yeast Extract (% YE) 0.5 > 0 2.0 > 0 2.0 > 0
Horse Blood (% HB) NA 2.5 > 0 2.5 > 0
pH adjustment to 7.8 NA Initially > NoAdj q1h > NoAdj
Incubation time (h) 15 > 18 > 24 12 > 15, 18, 24 3*, 5†, 6‡
Ideal dilution (Tube #) NA 5 > 4 > 1–3 6 = 7 = 8
  1. SM = Skim Milk; > implies "superior to"; NA = non applicable to that Phase; 17%G:TSB = 17% glycerol in trypticase soy broth; THB = Todd Hewitt Broth; BHI = Brain Heart Infusion; NoAdj= no adjustment; *Tube 6; †Tube 7; ‡Tube 8.