Comparison of three standard liquid media with a supplemented medium for culture of S. pneumoniae INS-E611. Five-hour growth curves for a PRSP strain in four different culture media: standard BHI (two brands), standard THB, and supplemented THB. All curves were obtained from Tube 7 (Phase 2). Bacterial growth was poor in standard BBL-BHI, and was not used for further experiments. As expected, there was no difference among these media when every data point along time was compared by repeated measures ANOVA (P = 0.7976). However, there was a significant difference in maximal growth between supplemented THB and standard media, as illustrated by Figure 2.