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Table 1 PCR oligonucleotides

From: An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants

Name

Sequence (5'→3')1

Gene-specific primers

 

G-UpF-GWL2

TACAAAAAAGCAGGCTccgatcagttgcaacacatc

G-UpR-Gm2

TCAGAGCGCTTTTGAAGCTAATTCGttgttcctgcaccaccatct

G-DnF-Gm2

AGGAACTTCAAGATCCCCAATTCGccggcgagttccacctgaa

G-DnR-GWR2

TACAAGAAAGCTGGGTggttgagcttgctgtcgata

Common primers

 

Gm-F

CGAATTAGCTTCAAAAGCGCTCTGA

Gm-R

CGAATTGGGGATCTTGAAGTTCCT

GW-attB 1

GGGGACAAGTTTGTACAAAAAAGCAGGCT

GW-attB 2

GGGGACCACTTTGTACAAGAAAGCTGGGT

  1. 1Sequences in capital letters are common for all genes amplified and overlap with the Gm or attB primer sequences. Lower-case letters indicate gene-specific sequences, here PA1520. PCR oligonucleotide sequences for all 27 genes amplified in this study are given in Table 2 (see Additional file 1).
  2. 2G, gene specific sequence, here PA1520.
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BMC Microbiology

ISSN: 1471-2180

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