Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts

BMC Microbiology

Table 1 Sequence characteristics of primers developed in this study.

Name	Sequence (5' to 3')	Nomenclature	Bases	Tm
NSA3	AAACTCTGTCGTGCTGGGGATA	nu-SSU-1543-5'	22	67
NSI1	GATTGAATGGCTTAGTGAGG	nu-SSU-1671-5'	20	59
58A1F	GCATCGATGAAGAACGC	nu-5.8S-2206-3'	17	61
58A2F	ATCGATGAAGAACGCAG	nu-5.8S-2208-3'	17	57
58A2R	CTGCGTTCTTCATCGAT	nu-5.8S-2192-5'	17	57
NLB3	GGATTCTCACCCTCTATGA	nu-LSU-2754-5'	19	56
NLB4	GGATTCTCACCCTCTATGAC	nu-LSU-2755-5'	20	57
NLC2	GAGCTGCATTCCCAAACAACTC	nu-LSU-2821-5'	22	67
Plant control primers
NSIP	GATTGAATGATCCGGTGAAG	PC-nu-SSU-1671-5'	20	62
NLBP	GCTGTCACCCTCTCAGGC	PC-nu-LSU-2755-5'	18	64

Sequences are all listed 5' to 3' for the oligonucleotides. Nomenclature after Gargas and DePriest [23] as extended in this study. Tm is calculated by nearest neighbour method as an on-line service provided by Sigma-Genosys (The Woodlands, TX).

ISSN: 1471-2180