Diagram of primer locations in the ribosomal cassette consisting of SSU, ITS1, 5.8S, ITS2, and LSU rDNA. Primers are positioned above (forward primers) or below (reverse) their sequence positions. ITS1, ITS2, ITS3, and ITS4 from White et al. , primers ITS8mun, ITS9mun, ITS10mun, NL5mun, NL6Amun, NL6Bmun, NL8mun from Egger , primers ITS1-F, ITS4-B from Gardes and Bruns  and the remaining primers (NSA3, NSI1, 58A1F, 58A2F, 58A2R, NLB4, NLC2) from this study. Scale is in base pairs according to the extension of the Gargas and DePriest  nomenclature system described in this study.