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Figure 7 | BMC Microbiology

Figure 7

From: HinT proteins and their putative interaction partners in Mollicutes and Chlamydiaceae

Figure 7

Immune co-precipitation. A. Affinity purified proteins, Cp265 (265C), Cp266 (266C), Cp267 (267C) and OppAC, were analyzed on 15 % SDS-polyacrylamide gels by Coomassie staining (C) or on Western blots by immunostaining with an anti-Protein C antibody (W). [35S]-labeled Cp265, Cp266 and Cp267 (Captives) were incubated at room temperature for 1 h with Cp265C, Cp266C, Cp267C and OppAC (Catcher). Immune complexes were precipitated with anti-Protein C-matrix, and the supernatants (B.) and precipitates (C.) analyzed on 15 % SDS-polyacrylamide gels by autoradiography using a phosphorimager. As a negative control, the [35S]-labeled proteins were subjected to the Protein C-matrix without a catcher (Sepha.αC). M, prestained low-molecular weight marker (Biorad)

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