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Figure 5 | BMC Microbiology

Figure 5

From: HinT proteins and their putative interaction partners in Mollicutes and Chlamydiaceae

Figure 5

The yeast-two hybrid assay. AH109 were co-transformed with the pGADT7 (AD) and pGBKT7 (BD) constructs containing hit L of M. hominis, Cp265, Cp266 or Cp267 from C. pneumoniae, pGADT7-T, expressing the SV40 large T-antigen and pGBKT7-53, expressing the murine p53 protein (K+), or pGADT7 and pGBKT7 without fusion partners (K-). Yeast cultures were incubated at 30°C on histidine-deficient agar plates for 4 days.

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