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Table 1 Strains and Plasmids

From: Role of core promoter sequences in the mechanism of swarmer cell-specific silencing of gyrB transcription in Caulobacter crescentus

Strain

 

Reference

E. coli

  

S17-1

Rp4-2, Tc::Mu, Km::Tn7

[49]

TG-1

F_, lacIq, proA+B+ lacZ _M15

[61]

C. crescentus

  

NA1000 syn-1000 (synchronizable strain)

 

[22]

JG3003 syn-1000, smc7 (spec resistance)

 

[this study]

JG3402 syn-1000, dps2 (gent resistance)

 

[this study]

Plasmids

 

Reference

pBluescript-KS+

cloning vector

[Stratagene]

placZ/290

pRK290 derivative containing a promoterless lacZ gene

[48]

pNPTS139

sacB counter selection vectors; (kanr)

[M.R.K. Alley]

pJEKS- gyrB500

500 bp, Pst I/Stu I gyrB fragment in pBluescript-KS+

[this study]

pJEKS-P3

904 bp Pst I/Hin dIII gyrB promoter fragment

[this study]

pJGZ1.65

1.65 kb Pst I/Eco RI fragment in placZ/290, gyrB-lacZ transcriptional reporter fusion

[17]

pJEZ1.1

1.3 kb Pst I/Hin dIII fragment in placZ/290

[this study]

pJEZP2

942 bp Pst I/Hin dIII gyrB-lacZ transcriptional reporter fusion

[this study]

pJEZP3

904 bp Pst I/Hin dIII gyrB-lacZ transcriptional reporter fusion

[this study]

pJEZP2s

167 bp Pst I/(native)Stu I gyrB-lacZ transcriptional reporter fusion

[this study]

pJEZP3s

132 bp Pst I/(native)Stu I gyrB-lacZ transcriptional reporter fusion

[this study]

pJEZP4

180 bp Pst I/Hin dIII gyrB-lacZ transcriptional reporter fusion

[this study]

pJEZP5

80 bp Pst I/Hin dIII gyrB-lacZ transcriptional reporter fusion

[this study]