Strain | Â | Reference |
---|---|---|
E. coli | Â | Â |
S17-1 | Rp4-2, Tc::Mu, Km::Tn7 | [49] |
TG-1 | F_, lacIq, proA+B+ lacZ _M15 | [61] |
C. crescentus | Â | Â |
NA1000 syn-1000 (synchronizable strain) | Â | [22] |
JG3003 syn-1000, smc7 (spec resistance) | Â | [this study] |
JG3402 syn-1000, dps2 (gent resistance) | Â | [this study] |
Plasmids | Â | Reference |
pBluescript-KS+ | cloning vector | [Stratagene] |
placZ/290 | pRK290 derivative containing a promoterless lacZ gene | [48] |
pNPTS139 | sacB counter selection vectors; (kanr) | [M.R.K. Alley] |
pJEKS- gyrB500 | 500 bp, Pst I/Stu I gyrB fragment in pBluescript-KS+ | [this study] |
pJEKS-P3 | 904 bp Pst I/Hin dIII gyrB promoter fragment | [this study] |
pJGZ1.65 | 1.65 kb Pst I/Eco RI fragment in placZ/290, gyrB-lacZ transcriptional reporter fusion | [17] |
pJEZ1.1 | 1.3 kb Pst I/Hin dIII fragment in placZ/290 | [this study] |
pJEZP2 | 942 bp Pst I/Hin dIII gyrB-lacZ transcriptional reporter fusion | [this study] |
pJEZP3 | 904 bp Pst I/Hin dIII gyrB-lacZ transcriptional reporter fusion | [this study] |
pJEZP2s | 167 bp Pst I/(native)Stu I gyrB-lacZ transcriptional reporter fusion | [this study] |
pJEZP3s | 132 bp Pst I/(native)Stu I gyrB-lacZ transcriptional reporter fusion | [this study] |
pJEZP4 | 180 bp Pst I/Hin dIII gyrB-lacZ transcriptional reporter fusion | [this study] |
pJEZP5 | 80 bp Pst I/Hin dIII gyrB-lacZ transcriptional reporter fusion | [this study] |