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Table 1 Strains and Plasmids

From: Role of core promoter sequences in the mechanism of swarmer cell-specific silencing of gyrB transcription in Caulobacter crescentus

Strain   Reference
E. coli   
S17-1 Rp4-2, Tc::Mu, Km::Tn7 [49]
TG-1 F_, lacIq, proA+B+ lacZ _M15 [61]
C. crescentus   
NA1000 syn-1000 (synchronizable strain)   [22]
JG3003 syn-1000, smc7 (spec resistance)   [this study]
JG3402 syn-1000, dps2 (gent resistance)   [this study]
Plasmids   Reference
pBluescript-KS+ cloning vector [Stratagene]
placZ/290 pRK290 derivative containing a promoterless lacZ gene [48]
pNPTS139 sacB counter selection vectors; (kanr) [M.R.K. Alley]
pJEKS- gyrB500 500 bp, Pst I/Stu I gyrB fragment in pBluescript-KS+ [this study]
pJEKS-P3 904 bp Pst I/Hin dIII gyrB promoter fragment [this study]
pJGZ1.65 1.65 kb Pst I/Eco RI fragment in placZ/290, gyrB-lacZ transcriptional reporter fusion [17]
pJEZ1.1 1.3 kb Pst I/Hin dIII fragment in placZ/290 [this study]
pJEZP2 942 bp Pst I/Hin dIII gyrB-lacZ transcriptional reporter fusion [this study]
pJEZP3 904 bp Pst I/Hin dIII gyrB-lacZ transcriptional reporter fusion [this study]
pJEZP2s 167 bp Pst I/(native)Stu I gyrB-lacZ transcriptional reporter fusion [this study]
pJEZP3s 132 bp Pst I/(native)Stu I gyrB-lacZ transcriptional reporter fusion [this study]
pJEZP4 180 bp Pst I/Hin dIII gyrB-lacZ transcriptional reporter fusion [this study]
pJEZP5 80 bp Pst I/Hin dIII gyrB-lacZ transcriptional reporter fusion [this study]