Figure 7

The gyrB promoter is expressed in the wild-type temporal pattern in smc and dps null strains. The plasmid pJEZP5, containing the gyrB promoter fused to a promoter-less copy of lacZ, was moved into NA1000 (wt, top), JG3003 (middle) and JG3402 (bottom) by conjugation. Transcription from the gyrB promoter was monitored during synchronized growth of the two strains by immunoprecipitation of β-galactosidase pulse-labeled at intervals during the cell cycle. For each strain, the protein labeled during each five-minute interval is shown beneath a diagram of the corresponding stage in the cell cycle, as determined by light microscopy.