Serotypes, intimin variants and other virulence factors of eae positive Escherichia coli strains isolated from healthy cattle in Switzerland. Identification of a new intimin variant gene (eae-η2)

Table 6 Sequences of oligonucleotide primers used for detection other virulence genes

Target	Primer	Oligonucleotide sequence (5'-3') Sequence	Product size	Reference
stx	VT1 VT2	ATTGAGCAAAATAATTTATAT GTG TGATGATGGCAATTCAGTAT	523 bp 520 bp	54
stx 1	KS7 KS8	CCCGGATCCATGAAAAAAACATTATTAATAGC CCCGAATTCAGCTATTCTGAGTCAACG	285 bp	55
stx 2	VT2-e VT2-f	AATACATTATGGGAAAGTAATA TAAACTGCACTTCAGCAAAT	348 bp	56
ehxA	HlyA1 HlyA2	GGTGCAGCAGAAAAAGTTGTAG TCTCGCCTGATAGTGTTTGGTA	311 bp	31
astA	EAST11a EAST11b	CCATCAACACAGTATATCCGA GGTCGCGAGTGACGGCTTTGT	111 bp	59
EAF	EAF1 EAF25	CAGGGTAAAAGAAAGATGATAA TATGGGGACCATGTATTATCA	397 bp	57
bfpA	EP1 EP2	AATGGTGCTTGCGCTTGCTGC GCCGCTTTATCCAACCTGGTA	326 bp	58

ISSN: 1471-2180