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Table 4 Expression profile of cytokine-related genes in epithelial cell lines exposed for 4 h to exogenous Tat protein (100 ng/ml).

From: Proliferative activity of extracellular HIV-1 Tat protein in human epithelial cells: expression profile of pathogenetically relevant genes

Gene name

GenBank accession No.

Description

Fold change in expression1

   

MCF-7 cell line mean ± SD

AV-3 cell line mean ± SD

Mean of the two cell lines

MIF

NM 002415

Macrophage migration inhibitory factor (glycosylation-inhibiting factor)

5.2 ± .9

2.1 ± 1.8

3.7

IL-19

NM 013371

Interleukin 19

4.0 ± 1.1

2.4 ± .3

3.2

IL-13RA1

NM 001560

Interleukin 13 receptor. alpha 1

4.1 ± 1.2

2.1 ± .6

3.1

GP130

NM 002184

IL-6 signal transducer (gp 130, oncostatin M receptor)

3.9 ± 1.4

2.0 ± .3

3.0

CCR3

NM 001837

Chemokine (CC motif) receptor 3

4.2 ± 1.1

1.6 ± .3

2.9

IL-11

M57765

Interleukin 11

3.9 ± .7

1.7 ± .4

2.8

SCYE1

NM 004757

Small inducible cytokine subfamily E. member 1 (endothelial monocyte-activating)

2.6 ± .5

3.0 ± .6

2.8

BMP2

NM 001200

Bone morphogenetic protein 2

3.4 ± .5

2.0 ± .5

2.7

IL-18

NM 001562

Interleukin 18 (interferon-gamma-inducing factor)

2.2 ± .6

3.1 ± .9

2.7

TGFb1

X02812

Transforming growth factor beta 1 (Camurati-Engelmann disease)

2.8 ± .5

2.1 ± .4

2.5

SCYC1

U23772

Chemokine (C motif) ligand 1

2.5 ± .6

1.8 ± .4

2.2

BMP1

NM 006128

Bone morphogenetic protein 1

2.7 ± 1.0

1.5 ± .5

2.1

VEGF-C

X94216

Vascular endothelial growth factor C

2.5 ± 1.1

1.6 ± .2

2.0

IL-15

AF031167

Interleukin 15

2.0 ± .5

2.0 ± .3

2.0

TNFR1

M33294

Tumor necrosis factor receptor superfamily. member 1A

2.3 ± .7

1.7 ± .5

2.0

VEGF-B

U48801

Vascular endothelial growth factor B

2.2 ± .5

1.8 ± .1

2.0

CXCR4

NM 003467

Chemokine (CXC motif) receptor 4

2.1 ± .5

1.5 ± .5

1.8

IL-6

M14584

Interleukin 6 (interferon, beta 2)

2.0 ± .6

1.6 ± .2

1.8

IL-10

M57627

Interleukin 10 (high-level expression in both cell lines)

1.9 ± .5

1.7 ± .4

1.8

LT-b

NM 002341

Lymphotoxin beta (TNF superfamily, member 3)

1.8 ± .6

1.6 ± .8

1.7

PDGFa

X06374

Platelet-derived growth factor alpha polypeptide

1.7 ± .7

1.7 ± .5

1.7

LTbR

L04270

Lymphotoxin beta receptor (TNFR superfamily, member 3)

1.7 ± .2

1.6 ± .4

1.7

SCYA5 / RANTES

NM 002985

Chemokine (CC motif) ligand 5

1.6 ± .3

1.7 ± .5

1.6

IL-20

NM 018724

Interleukin 20

1.6 ± .7

1.6 ± .5

1.6

  1. 1. Mean densitometric values are expressed for each gene as fold difference in cultures exposed to Tat / control cultures not exposed to Tat. Negative (pUC18) and positive controls (GAPDH, cyclophillin A, RPL13A, beta-actin) were used to normalize the data. Data are reported only for genes that were up-regulated by Tat at least 1.5-fold in both cell lines.
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BMC Microbiology

ISSN: 1471-2180

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