Table 3 Expression profile of cell cycle-related genes in epithelial cell lines exposed for 4 h to exogenous Tat protein (100 ng/ml).
Gene name | GenBank accession No. | Description | Fold change in expression1 | ||
|---|---|---|---|---|---|
|  |  |  | MCF-7 cell line mean ± SD | AV-3 cell line mean ± SD | Mean of the two cell lines |
P21Waf1 (p21Cip1) | L47233 | Cyclin-dependent kinase inhibitor 1A (p21, Cip1) | 4.2 ± .8 | 5.0 ± .8 | 4.6 |
Cdk7 | NM 001799 | Cyclin-dependent kinase 7 | 3.8 ± .9 | 5.2 ± .7 | 4.5 |
Cul1 | NM 003592 | Cullin 1 | 2.7 ± .6 | 5.3 ± 1.1 | 4.0 |
Cullin-Cul3 | NM 003590 | Cullin 3 | 2.6 ± .7 | 5.3 ± 1.3 | 3.9 |
CDC37 | U63131 | Cell division cycle 37 (S. cerevisiae) homolog | 1.9 ± .3 | 5.9 ± .9 | 3.9 |
cyclinD1 | M64349 | Cyclin D1 (PRAD1 parathyroid adenomatosis 1) | 1.8 ± .3 | 5.8 ± .7 | 3.8 |
Cks1p9 | NM 001826 | CDC28 protein kinase 1 | 2.4 ± .5 | 4.9 ± 1.2 | 3.7 |
Cullin-Cul4A | AF077188 | Cullin 4A | 2.2 ± .4 | 4.8 ± 1.1 | 3.5 |
Cks2 | NM 001827 | CDC28 protein kinase 2 | 1.6 ± .5 | 5.2 ± 1.1 | 3.4 |
CDC45-like1 | NM 003504 | Cell division cycle 45-like (S. cerevisiae) | 5.1 ± 1.4 | 1.6 ± .4 | 3.4 |
CyclinH | U11791 | Cyclin H | 4.0 ± .6 | 2.6 ± 1.0 | 3.3 |
p27Kip1 | U10906 | Cyclin-dependent kinase inhibitor 1B (p27, Kip1) | 1.8 ± .2 | 4.8 ± 1.0 | 3.3 |
Cdk8 | X85753 | Cyclin-dependent kinase 8 | 1.6 ± .3 | 4.8 ± .6 | 3.2 |
CDC34 | L22005 | Ubiquitin-conjugating enzyme, cell division cycle 34 | 3.0 ± .5 | 3.3 ± .6 | 3.1 |
Chk1 | AF016582 | CHK1 (checkpoint, S. pombe) homolog | 1.6 ± .3 | 4.7 ± .9 | 3.1 |
Cyclin G | X77794 | Cyclin G1 | 3.2 ± .5 | 3.0 ± .3 | 3.1 |
CyclinG2 | L49506 | Cyclin G2 | 2.7 ± .6 | 3.3 ± .5 | 3.0 |
Cdc27 | NM 001256 | Cell division cycle 27 | 2.7 ± .8 | 3.2 ± .7 | 3.0 |
p18 | U17074 | p18 (cdk4 inhibitor) cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4) | 2.3 ± .5 | 3.6 ± 1.0 | 3.0 |
E2F | U47677 | Transcription factor 1 | 3.8 ± .9 | 1.9 ± .6 | 2.9 |
RAD17 | NM 002873 | RAD17 (S. pombe) homolog | 2.3 ± 1.0 | 3.3 ± .8 | 2.8 |
RAD50 | U63139 | RAD50 (S. cerevisiae) homolog | 2.4 ± .4 | 3.1 ± .8 | 2.8 |
Cullin-Cul2 | U83410 | Cullin 2 | 1.5 ± .5 | 3.8 ± .8 | 2.7 |
E2F-6 | AF059292 | E2F transcription factor 6 | 2.9 ± .6 | 2.3 ± .8 | 2.6 |
cyclinB2 | NM 004701 | Cyclin B2 | 1.8 ± .8 | 3.2 ± .5 | 2.5 |
CyclinF | U17105 | Cyclin F | 3.5 ± .8 | 1.5 ± .6 | 2.5 |
PRC1 | NM 003981 | Protein regulator of cytokinesis1 | 2.0 ± .4 | 3.0 ± .5 | 2.5 |
Cullin-Cul4B | AB014595 | Cullin 4B | 2.0 ± .3 | 2.9 ± .3 | 2.5 |
cyclinE1 | M73812 | Cyclin E1 | 2.0 ± .4 | 2.9 ± .3 | 2.5 |
Hus1 | NM 004507 | HUS1 (S. pombe) checkpoint homolog | 1.8 ± .8 | 3.1 ± .2 | 2.5 |
MPP2 | U74613 | Human putative M phase phosphoprotein 2 (MPP2) mRNA | 1.8 ± .6 | 3.1 ± .6 | 2.5 |
Ki67 (MKI67) | X65550 | Antigen identified by monoclonal Ab Ki-67 | 1.5 ± .3 | 3.3 ± .4 | 2.4 |
Skp1 | U33760 | Cyclin A / CDK2-associated p19 (Skp1) | 1.5 ± .3 | 3.2 ± .6 | 2.4 |
Skp2 | U33761 | Human cyclin A / CDK2-associated p45 (Skp2) | 2.0 ± .3 | 2.7 ± .6 | 2.4 |
PCNA | J04718 | Proliferating cell nuclear antigen | 1.9 ± .5 | 2.8 ± .5 | 2.4 |
Bax | L22474 | Bcl2-associated X protein | 1.6 ± .5 | 3.0 ± .7 | 2.3 |
cyclinE2 | NM 004702 | Cyclin E2 | 1.5 ± .4 | 3.1 ± .3 | 2.3 |
CyclinB | M25753 | Cyclin B1 | 1.7 ± .3 | 3.1 ± .5 | 2.4 |
CyclinC | M74091 | G1/S-specific Cyclin C | 1.6 ± .4 | 3.0 ± .6 | 2.3 |
MCM5 (CDC46) | NM 006739 | Minichromosome maintenance deficient (S. cerevisiae) 5 (cell division cycle 46) | 1.8 ± .2 | 2.7 ± .4 | 2.3 |
MRE11A | U37359 | Meiotic recombination (S. cerevisiae) 11 homolog A | 1.5 ± .7 | 2.9 ± .5 | 2.2 |
Cdk6 | X66365 | Cyclin-dependent kinase 6 | 2.0 ± .3 | 2.3 ± .9 | 2.2 |
Nibrin | AF058696 | Nijmegen breakage syndrome 1 (nibrin) | 1.8 ± .4 | 2.5 ± .5 | 2.2 |
Rbx1 | NM 014248 | Homo sapiens ring-box protein 1 (RBX1) mRNA | 1.5 ± .7 | 2.8 ± .6 | 2.2 |
MCM2 | D83987 | Minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin) | 1.5 ± .3 | 2.7 ± .3 | 2.1 |
MCM4 (CDC21) | X74794 | Minichromosome maintenance deficient (S. cerevisiae) 4 | 2.0 ± .4 | 1.8 ± .2 | 1.9 |
MCM7 (cdc47) | D28480 | Minichromosome maintenance deficient (S. cerevisiae) 7 | 1.5 ± .5 | 2.3 ± .7 | 1.9 |
c-abl | X16416 | V-abl Abelson murine leukemia viral oncogene homolog 1 | 1.6 ± .5 | 1.9 ± .6 | 1.7 |
Mdm2 | Z12020 | Mouse double minute 2, human homolog of p53-binding protein | 1.6 ± .6 | 1.8 ± .6 | 1.7 |
Rpa | L07493 | Replication protein A3 (14 Kd) | 1.6 ± .5 | 1.8 ± .4 | 1.7 |