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Figure 1 | BMC Microbiology

Figure 1

From: Chlamydia pneumoniae induces aponecrosis in human aortic smooth muscle cells

Figure 1

Diverse morphology of Cpn infection. HASMC (A, B, C and D) and HEp-2 cells (E) were infected for 72 h with Cpn-K6 at 128 IFU/cell (HASMC; A, B), Cpn-VR1310 at 8 IFU/cell (HASMC; C, D) and Cpn-K6 at 5 IFU/cell (HEp-2; E), respectively. Bacteria localized on the cell surface are stained with FITC (green), Chlamydia internally localized are stained with Texas Red (red) and FITC (for detail see material and methods). DNA of bacteria and host cells is stained with DAPI (blue). N = nucleus. Left row: extracellular Cpn. Middle row: extra- and intracellular Cpn. Right row: overlay of left- and middle row and DAPI staining. A, C: spot like infection of a HASMC; note that the majority of bacteria are localized intracellularly B, D: HASMC with a Cpn inclusion. E: Hep-2 cells with Cpn inclusions.

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