Table 1 List of primers used in this study. All sequences are written from 5' to 3'.
From: Crosstalk regulation among group 2- Sigma factors in Synechocystis PCC6803
Primer | Sequence |
|---|---|
sigB-1 | CGGAATTCTTGGGTATCTTTTTAGC |
sigB-2 | CCGAAGCTTGGGCAACTAACTGGC |
sigC-1 | CGGAATTCAAAGCCTGCCATCGGCC |
sigC-2 | CCGAAGCTTGTGGCCTAACCCAAATTTTC |
sigD-1 | CGGAATTCGAGTATGTGCTTCACAA |
sigD-2 | CCGAAGCTTCTTTTCTTTAGCTAGCT |
sigE-1 | CGGAATTCTTTGGAAAATCAATGACA |
sigE-2 | CCGAAGCTTGGTATCTATAACCAACC |
cat-1 | CGGGATCCCGCCCGGGAATTACGCCCCGCCC |
cat-2 | CGGGATCCCGCCCGGGCAGGAGCTAAGGAAGCTA |
rpoA-RT | TAACCTA |
sigB, C-RT | TAACCTT |
sigA, D, E-RT | TAACCCT |
glnN-RT | CCATCCGTC |
sigA forward | TGGAGTTGGAAACCGCC |
sigA reverse | GACTGCACCATTTTGTCTTTGG |
sigB forward | AGAAATGGCCCGCTATCCC |
sigB reverse | GCCCGCAGTTGATAAAGCC |
sigC forward | TGGAGTTGGAAACCGCC |
sigC reverse | GACTGCACCATTTTGTCTTTGG |
sigD forward | GATGGCCCTGCTGGAGC |
sigD reverse | TTGCGCTTCTGATATTTCTTGG |
sigE forward | CGGGCCGCAGAATCC |
sigE reverse | CCAACTCCGCCCATCG |
rpoA forward | GAGTTCGCCACTATTCTAGGCG |
rpoA reverse | TTAGGATCAATAACCTCCACCTC |
glnN forward | GATTTAACCAAGGACGCTGGC |
glnN reverse | CAAAACGGTTACCAGTGAAGGC |