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Figure 5 | BMC Microbiology

Figure 5

From: Alkane-induced expression, substrate binding profile, and immunolocalization of a cytochrome P450 encoded on the nifD excision element of Anabaena 7120

Figure 5

Ultrastructure and immunocytochemical detection of CYP110 in alkane treated Anabaena 7120. Nitrogen-fixing cultures were grown with shaking in continuous light at 30 C with ambient CO2. Test cultures were treated with 0.2% dodecane or 0.2% hexadecane for 30 days. (a) TEM of untreated vegetative cell, (b) TEM of dodecane-treated vegetative cell, (c) TEM of hexadecane-treated vegetative cell, (d) untreated control vegetative cell incubated with anti-CYP110 antiserum, (e) dodecane-treated vegetative cell incubated with anti-Cyp111 antiserum, (f) hexadecane-treated vegetative cell incubated with anti-CYP110 antiserum, (g) hexadecane treated heterocyst incubated with anti-CYP110 antiserum, (h) dodecane-treated vegetative cell incubated with non-immune rabbit IgG, (i) dodecane-treated vegetative cell incubated with anti-CYP110 antiserum previously adsorbed with purified CYP110.

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