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Figure 3 | BMC Microbiology

Figure 3

From: Alkane-induced expression, substrate binding profile, and immunolocalization of a cytochrome P450 encoded on the nifD excision element of Anabaena 7120

Figure 3

Expression of CYP110 protein in Anabaena 7120. Proteins were fractionated on 12% polyacrylamide gels, blotted to nitrocellulose and probed with rabbit anti-CYP110 antibody. Panel A displays samples treated with 0.2 % n-hexadecane. Panel B, control untreated samples taken at the same time points. Lanes 1 contain 600 ng of purified recombinant CYP110 protein. Lanes 2 are blank. Lanes 3 through 7 contain 50 μg of crude protein taken at 0, 2, 12, 24, and 36 hours. Panel C shows the densitometry trace volumes for the CYP110 protein in Panel A. Panel D is a Western blot showing cyp110-reactive proteins from the 36 hour hexadecane time point after separation into the soluble and membrane fractions. Lane 1: 50 μg of the cytosolic fraction and lane 4: 50 μg of the membrane fraction.

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BMC Microbiology

ISSN: 1471-2180

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