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Table 3 Patterns of restriction digestion of short 16S-23S intergenic spacer of lactobacilli and other bacteria isolated from different sources

From: Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling

Isolate Sph I Nco I Nhe I Ssp I Sfu I Eco RV Dra I Vsp I Hinc II Eco RI Hind III Identification
Chicken gastrointestinal tract
F 5.2 F 5.3 + - - + + - - + + - - Lact. plantarum b
F 5.7 - + - - - + - - - + + Lact. crispatus
Reg 24 25 26 - - - - - + - - - - - Lact. johnsonii a
Reg 32 - - - - - + - - - - - Lact. gasseri a
Reg 34 + + - - - - - + - - - Lact. reuteri
38 90 110 + + - - - - - + - - + Lact. vaginalis
98, 108 - - - - - + + - - - + Lact. rhamnosus
115 - + - - - - - - - + + Lact. acidophilus
'Coalho' cheese
AB2 - - - - - + + + - - + Lact. casei
AB3 + - - - - - - + - - - Lact. fermentum
AQ1 - - - - - + + - - - + Lact. rhamnosus
LV3 - + - - - - - - - + + Lact. acidophilus
CM3 - - - + - - + - - - + W. confusa
AB1 BL1 SM4 - - - + - - - - - - - Strep. thermophilus
CM2M17 - - - - - - + - - - - L. lactis
AQ2 CM4 LV1 - - - + - - - - - - - Ent. faecalis b
AQ2AT1 + - + + + - + + + + + Staph. aureus
Cassava starch sour fermentation
P16.01 P25.01 + - - - - - - + - - - Lact. fermentum
P24.03 P25.04 + - - + + - - + + - - Lact. plantarum b
P24.01 P29.01 + - - + - - - + + - - Lact. plantarum a
P10.06 + - - - - - - + - - + Lact. brevis
P10.02 - - - + - - + + + - - Lact. salivarius
P18.04 - - - - - + - - - - + Lact. perolens
Human sources
UFV H2b20 - + - - + - - - - - - Lact. delbrueckii
117 - - - - - + + - - - + Lact. rhamnosus
A15 A91 - - - + + - + - - - - Lact. ruminis
PM1 - - - - - + - - - - - Lact. johnsonii a
PM2 PM3 - - - - - + - - - - - Lact. jensenii a
  1. aSpecies identified by additional restriction digestion with Hind III, Bgl II and Pvu II of long spacer regions
  2. bSpecies identified by amplicons sizes