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Table 2 Theoretical patterns of restriction digestion of short 16S-23S intergenic spacer in different species of Bacteroides, Bifidobacterium, Carnobacterium, Enterococcus, Escherichia, Lactococcus, Leuconostoc, Oenococcus, Pediococcus, Propionibacterium, Staphylococcus, Streptococcus, and Weissella

From: Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling

  Sph I Nco I Nhe I Ssp I Sfu I Eco RV Dra I Vsp I Hinc II Eco RI Avr II Hind III
Bact. fragilis - - - - + - - - + - - -
B. longum - - - - + - - - - + - -
B. lactis - - - - - - - - - - - -
C. piscicola - - - + - - ++a - - - - +
C. gallinarum - - - - - - ++ - - + - +
C. mobile - - - - - - - - - + - -
Ent. faecalis - - - + - - - - - - - -
Ent. faecium - - - + - - - - - - - -
Ent. raffinosus - - - + - - - - - - - +
E. coli - + - - + - + - - - + -
L. lactis - - - - - - + - - - + -
Leuc. mesenteroides - - + - - - ++ - - - - +
O. oeni - - - - + - + - - - - +
Ped. acidilacitii + - + - - - + ++ - - - +
Ped. parvulus + - + - - - + - - - - +
P. freudenreichii - - - - - - - - - - + -
Staph. aureus + - + + + - +++ + + + - ++
Strep. thermophilus - - - + - - - - - - - -
W. confusa - - - + - - +++ - - - - +
W. paramesenteroides - - - - - - +++ - - - - -
  1. aTwo or more restriction sites found into spacer region