Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling

BMC Microbiology

Table 2 Theoretical patterns of restriction digestion of short 16S-23S intergenic spacer in different species of Bacteroides, Bifidobacterium, Carnobacterium, Enterococcus, Escherichia, Lactococcus, Leuconostoc, Oenococcus, Pediococcus, Propionibacterium, Staphylococcus, Streptococcus, and Weissella

	Sph I	Nco I	Nhe I	Ssp I	Sfu I	Eco RV	Dra I	Vsp I	Hinc II	Eco RI	Avr II	Hind III
Bact. fragilis	-	-	-	-	+	-	-	-	+	-	-	-
B. longum	-	-	-	-	+	-	-	-	-	+	-	-
B. lactis	-	-	-	-	-	-	-	-	-	-	-	-
C. piscicola	-	-	-	+	-	-	++^a	-	-	-	-	+
C. gallinarum	-	-	-	-	-	-	++	-	-	+	-	+
C. mobile	-	-	-	-	-	-	-	-	-	+	-	-
Ent. faecalis	-	-	-	+	-	-	-	-	-	-	-	-
Ent. faecium	-	-	-	+	-	-	-	-	-	-	-	-
Ent. raffinosus	-	-	-	+	-	-	-	-	-	-	-	+
E. coli	-	+	-	-	+	-	+	-	-	-	+	-
L. lactis	-	-	-	-	-	-	+	-	-	-	+	-
Leuc. mesenteroides	-	-	+	-	-	-	++	-	-	-	-	+
O. oeni	-	-	-	-	+	-	+	-	-	-	-	+
Ped. acidilacitii	+	-	+	-	-	-	+	++	-	-	-	+
Ped. parvulus	+	-	+	-	-	-	+	-	-	-	-	+
P. freudenreichii	-	-	-	-	-	-	-	-	-	-	+	-
Staph. aureus	+	-	+	+	+	-	+++	+	+	+	-	++
Strep. thermophilus	-	-	-	+	-	-	-	-	-	-	-	-
W. confusa	-	-	-	+	-	-	+++	-	-	-	-	+
W. paramesenteroides	-	-	-	-	-	-	+++	-	-	-	-	-