Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling

Table 1 Theoretical patterns of restriction digestion of short 16S-23S intergenic spacer in different species of Lactobacillus

	Sph I	Nco I	Nhe I	Ssp I	Sfu I	Eco RV	Dra I	Vsp I	Hinc II	Eco RI	Hind III
Lact. delbrueckii	-^a	+^a	-	-	+	-	-	-	-	-	-
Lact. amylolyticus	-	+	-	-	+	-	-	-	-	-	+
Lact. acidophilus	-	+	-	-	-	-	-	-	-	+	+
Lact. amylovorus	-	+	-	-	-	-	-	-	-	+	+
Lact. helveticus	-	+	-	-	-	-	-	-	-	+	+
Lact. crispatus	-	+	-	-	-	+	-	-	-	+	+
Lact. perolens	-	-	-	-	-	+	-	-	-	-	+
Lact. gasseri	-	-	-	-	-	+	-	-	-	-	-
Lact. johnsonii	-	-	-	-	-	+	-	-	-	-	-
Lact. jensenii	-	-	-	-	-	+	-	-	-	-	-
Lact. reuterii	+	+	-	-	-	-	-	+	-	-	-
Lact. vaginalis	+	+	-	-	-	-	-	++^b	-	-	+
Lact. brevis	+	-	-	-	-	-	-	+	-	-	+
Lact. sakei	+	-	-	-	-	-	++	-	-	-	+
Lact. casei	-	-	-	-	-	+	+	+	-	-	+
Lact. rhamnosus	-	-	-	-	-	+	+	-	-	-	+
Lact. salivarius	-	-	-	+	-	-	++	+	+	-	-
Lact. ruminis	-	-	-	+	+	-	+	-	-	-	-
Lact. plantarum a	+	-	-	+	-^c	-	-	+	+	-	-
Lact. plantarum b	+	-	-	+	+^c	-	-	+	+	-	-
Lact. fermentum	+	-	-	-	-	-	-	+	-	-	-

^aPlus and minus signals represent positive and negative restriction digestion, respectively
^bTwo or more restriction sites found into spacer region
^cPolymorphic site present in some strains of Lact. plantarum and absent in others

ISSN: 1471-2180