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Table 1 Theoretical patterns of restriction digestion of short 16S-23S intergenic spacer in different species of Lactobacillus

From: Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling

  Sph I Nco I Nhe I Ssp I Sfu I Eco RV Dra I Vsp I Hinc II Eco RI Hind III
Lact. delbrueckii -a +a - - + - - - - - -
Lact. amylolyticus - + - - + - - - - - +
Lact. acidophilus - + - - - - - - - + +
Lact. amylovorus - + - - - - - - - + +
Lact. helveticus - + - - - - - - - + +
Lact. crispatus - + - - - + - - - + +
Lact. perolens - - - - - + - - - - +
Lact. gasseri - - - - - + - - - - -
Lact. johnsonii - - - - - + - - - - -
Lact. jensenii - - - - - + - - - - -
Lact. reuterii + + - - - - - + - - -
Lact. vaginalis + + - - - - - ++b - - +
Lact. brevis + - - - - - - + - - +
Lact. sakei + - - - - - ++ - - - +
Lact. casei - - - - - + + + - - +
Lact. rhamnosus - - - - - + + - - - +
Lact. salivarius - - - + - - ++ + + - -
Lact. ruminis - - - + + - + - - - -
Lact. plantarum a + - - + -c - - + + - -
Lact. plantarum b + - - + +c - - + + - -
Lact. fermentum + - - - - - - + - - -
  1. aPlus and minus signals represent positive and negative restriction digestion, respectively
  2. bTwo or more restriction sites found into spacer region
  3. cPolymorphic site present in some strains of Lact. plantarum and absent in others