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Figure 2 | BMC Microbiology

Figure 2

From: Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling

Figure 2

Restriction digestion of the three 16S-23S intergenic spacers of Lactobacillus acidophilus ATCC 4356. PCR amplification of rRNA spacer regions was done and amplicons were cleaved with 11 restriction enzymes and fragments separated by agarose gel electrophoresis at 100 V for 45 min. Plus and minus signs mean positive or negative cleavage of long, medium or short PCR amplicon, respectively. GibcoBRL 1 kb DNA ladder was used for sizing DNA fragments and molecular masses are shown at right in bp.

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